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Analysis of genetic diversity in the bioenergy plant Erianthus arundinaceus (Poaceae: Andropogoneae) using amplified fragment length polymorphism markers.

机译:使用扩增的片段长度多态性标记分析生物能源植物赤莲(禾本科:Andropogoneae)的遗传多样性。

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摘要

The genetic diversity among 30 accessions including 18 Erianthus arundinaceus and 12 Saccharum species was analyzed using amplified fragment length polymorphism (AFLP) markers. We obtained 2245 fragments using 16 primer combinations. The Erianthus collection generated 1115 fragments of which 772 (69.2%) were polymorphic. Of the 342 monomorphic fragments (30.8%), 222 were specific to E. arundinaceus. The neighbor-joining tree based on the detected fragments clearly separated the Erianthus and Saccharum accessions into distinct groups. The Erianthus collection was further subdivided into two Japanese groups and one Indonesian group, with a high level of genetic differentiation among these groups (Fst=0.2062). These results represent the first molecular characterization of the Japanese E. arundinaceus breeding materials and provide a useful initial resource for breeding to improve the bioenergy potential of Erianthus spp.
机译:使用扩增片段长度多态性(AFLP)标记分析了包括18个Erianthus arundinaceus和12个糖种在内的30个种质之间的遗传多样性。我们使用16个引物组合获得了2245个片段。 Erianthus集合生成了1115个片段,其中772个(69.2%)是多态的。在342个单态片段(30.8%)中,有222个特异于阿鲁迪纳斯大肠杆菌。基于检测到的片段的邻居加入树清楚地将Erianthus和Saccharum种质分成不同的组。 Erianthus集合进一步细分为两个日本组和一个印度尼西亚组,这些组之间的遗传分化水平很高(F st = 0.2062)。这些结果代表了日本E. arundinaceus育种材料的第一个分子特征,并为育种提供了有用的初始资源,以提高Erianthus spp的生物能潜力。

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