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首页> 外文期刊>Biochemistry >A functional spliced-variant of beta 2 subunit of Kv1 channels in C6 glioma cells and reactive astrocytes from rat lesioned cerebellum.
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A functional spliced-variant of beta 2 subunit of Kv1 channels in C6 glioma cells and reactive astrocytes from rat lesioned cerebellum.

机译：C6胶质瘤细胞和来自大鼠小脑的反应性星形胶质细胞中Kv1通道的beta 2亚基的功能性剪接变体。

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摘要

Voltage-gated K(+) channels (Kv1) are important in glia, being required for cell proliferation. Herein, reactive astrocytes from a rat cerebellar lesion were shown to contain Kv1.1, -1.2, -1.3, -1.4, and -1.6 alpha plus beta1.1 subunits, as well as an unusual beta2.1 constituent; the latter was also found in a glioblastoma C6 cell line, together with Kv1.1, -1.3, and -1.6 and beta1.1 subunits. Reverse transcriptase-polymerase chain reaction revealed a novel product of the beta2 gene in C6 cells and reactive astrocytes, but not in cultured astrocytes or rat normal brain. Its cloning identified a variant, Kvbeta2.1A, alternatively spliced between I24 and Y39. Despite this 14 residue deletion, Kvbeta2.1A assembled cotranslationally with Kv1.1 or -1.2 and, when coexpressed with Kv1. 4 in oocytes, increased the inactivation rate of this K(+) current. Whereas the full-length beta2.1 gave a large increase in the amplitude of the Kv1.1 current in oocytes, the effect of beta2.1A varied from a modest elevation of the current to a slight suppression in some cases. In summary, this is the first report of the existence of an alternatively spliced product of the Kvbeta2.1 gene in C6 cells and reactive astrocytes, and supports the involvement of its core region (residues 39 onward) in assembly with alpha subunits while excluding a contribution of the adjacent 14 residues to accelerating the inactivation of Kv1.4.
机译：电压门控的K（+）通道（Kv1）在胶质细胞中很重要，是细胞增殖所必需的。在此，来自大鼠小脑病变的反应性星形胶质细胞显示含有Kv1.1，-1.2，-1.3，-1.4和-1.6 alpha加beta1.1亚基，以及不寻常的beta2.1成分;后者也在胶质母细胞瘤C6细胞系中以及Kv1.1，-1.3，-1.6和beta1.1亚基中发现。逆转录酶-聚合酶链反应显示在C6细胞和反应性星形胶质细胞中有一个beta2基因的新产物，但在培养的星形胶质细胞或大鼠正常脑中却没有。其克隆鉴定出变体Kvbeta2.1A，其可变剪接在I24和Y39之间。尽管删除了这14个残基，但Kvbeta2.1A与Kv1.1或-1.2共翻译组装，并且在与Kv1共表达时。卵母细胞中的4，增加了这种K（+）电流的失活率。全长beta2.1使卵母细胞中Kv1.1电流的幅度大大增加，而beta2.1A的作用从电流的适度升高变化到某些情况下的轻微抑制。总之，这是关于Cv6细胞和反应性星形胶质细胞中Kvbeta2.1基因的选择性剪接产物存在的第一份报告，并支持其核心区域（残基39起）参与与α亚基组装的过程，而排除了相邻的14个残基对Kv1.4失活的加速作用。

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