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首页> 外文期刊>Glia >Heterogeneity of Kir4.1 channel expression in glia revealed by mouse transgenesis
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Heterogeneity of Kir4.1 channel expression in glia revealed by mouse transgenesis

机译：小鼠转基因揭示了胶质细胞中Kir4.1通道表达的异质性

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摘要

The weakly inwardly rectifying K~+ channel Kir4.1 is found in many glial cells including astrocytes. However, questions remain regarding the relative contribution of Kir4.1 to the resting K~+ conductance of mature astrocytes in situ. We employed a bacterial artificial chromosome transgenic approach in mice to visualize Kir4.1 expression in vivo. These mice (Kir4.1-EGFP) express enhanced green fluorescent protein (EGFP) under the transcriptional control of the Kir4.1 promoter. The brains of adult Kir4.1-EGFP transgenic mice showed co-expression of EGFP and Kir4.1 in astrocytes. In addition, weaker expression of EGFP was detected in NG2+ glial cells when compared with EGFP expression in GFAP+ glial cells. Whole-cell voltage clamp recordings of EGFP+ glial cells in the CA1 area of the adult mouse hippocampus indicated astrocytes displaying properties consistent with both the "passive" and "complex" subpopulations. EGFP+ cells with bright fluorescence had the linear current-voltage (I-V) relationships and extensive gap junctional coupling characteristic of passive astrocytes. However, EGFP+ glia with weaker fluorescence displayed properties associated with complex astrocytes including nonlinear I-V relationships and lack of intercellular gap junctional coupling. Pharmacological blockade of inward currents implied that Kir4.1 channels constitute the dominant resting K+ conductance in both glial cell types and are more highly expressed in passive astrocytes. These results suggest differential expression of Kir4.1 in glia and that this channel likely underlies the resting K~+ conductance in passive and complex astrocytes.
机译：在包括星形胶质细胞在内的许多神经胶质细胞中发现了弱向内整流的K〜+通道Kir4.1。然而，关于Kir4.1对成熟星形胶质细胞原位静息K +电导的相对贡献仍然存在疑问。我们在小鼠中采用了细菌人工染色体转基因方法，以在体内可视化Kir4.1表达。这些小鼠（Kir4.1-EGFP）在Kir4.1启动子的转录控制下表达增强的绿色荧光蛋白（EGFP）。成年Kir4.1-EGFP转基因小鼠的大脑在星形胶质细胞中共表达EGFP和Kir4.1。此外，与GFAP +胶质细胞中的EGFP表达相比，NG2 +胶质细胞中EGFP的表达较弱。成年小鼠海马CA1区EGFP +神经胶质细胞的全细胞电压钳记录表明，星形胶质细胞显示出与“被动”和“复杂”亚群一致的特性。具有明亮荧光的EGFP +细胞具有线性电流-电压（I-V）关系和被动星形胶质细胞的广泛间隙连接偶联特性。但是，具有较弱荧光的EGFP +胶质细胞显示出与复杂星形胶质细胞相关的特性，包括非线性I-V关系和缺乏细胞间间隙连接偶联。内向电流的药理学阻断表明，Kir4.1通道在两种神经胶质细胞类型中均构成主要的静息K +电导，并且在被动星形胶质细胞中表达更高。这些结果表明Kir4.1在胶质细胞中的差异表达，并且该通道可能是被动和复杂星形胶质细胞中静息K +电导的基础。

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