• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Islets >Augmented damage of islets by impaired exocrine acinar cells undergoing apoptosis that is possibly converted to necrosis during isolation.
【24h】

Augmented damage of islets by impaired exocrine acinar cells undergoing apoptosis that is possibly converted to necrosis during isolation.

机译：受损的外分泌腺细胞经历凋亡会增强胰岛的损害，凋亡可能在分离过程中转化为坏死。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Islet damage attributed to impaired exocrine cells during pancreas preservation and isolation procedure remains elusive, although released exocrine enzymes could directly damage islets. The aim of this study is to investigate the cellular mechanisms associated with exocrine cells and their possible impact on the islet cell survival and function after isolation. Mouse pancreata were stored in cold University of Wisconsin preservation solution for 0, 24 and 48 h and incubated with or without collagenase at 37 degrees C for 15 min. During preservation, the percentage of exocrine cells with necrosis, which means impaired cellular membrane that allows intracellular enzymes to be released, remains low (< 10%) regardless of preservation time; whereas the percentage of exocrine cells with apoptosis, which means impaired nucleus and possible intact cellular membrane, increases over time of preservation. After collagenase-free incubation, however, the percentage of exocrine cells with necrosis became higher in longer preservation time, and more than 60% of the necrotic exocrine cells contained apoptosis as well. Islet cells located in pancreata with intact structure are almost kept away either from necrotic or apoptotic changes even after 48 h preservation followed by collagenase-free incubation. However, when islets are isolated after collagenase-containing incubation, the percentage of islet cells with necrosis increases over time of preservation up to approximately 40%. This study suggests that exocrine cells with necrosis could cause damage of isolated islets when the pancreas is dissociated and that the necrosis in exocrine cells might be induced mainly as the conversion from apoptosis that has already existed during preservation.
机译：尽管在胰腺保存和分离过程中外分泌细胞受损导致的胰岛损害仍然难以捉摸，尽管释放的外分泌酶可能直接损害胰岛。这项研究的目的是研究与外分泌细胞相关的细胞机制及其对分离后胰岛细胞存活和功能的可能影响。将小鼠胰腺保存在威斯康星大学的冷保存溶液中0、24和48小时，并在有或没有胶原酶的情况下于37摄氏度孵育15分钟。在保存过程中，外分泌细胞坏死的百分比保持较低（<10％），而坏死意味着细胞膜被破坏，允许细胞内酶释放，细胞膜受损。而具有凋亡功能的外分泌细胞的百分比随保存时间的延长而增加，这意味着细胞核受损和可能完整的细胞膜。然而，在无胶原酶的温育后，具有更长坏死时间的坏死外分泌细胞的百分比变得更高，并且超过60％的坏死外分泌细胞也含有凋亡。即使保存48小时后再进行无胶原酶孵育，位于胰脏中具有完整结构的胰岛细胞也几乎远离坏死或凋亡变化。但是，如果在含胶原酶的孵育后分离出胰岛，则具有坏死作用的胰岛细胞的百分比会随着保存时间的增加而增加，最高可达约40％。这项研究表明，胰腺被解离时，具有坏死作用的外分泌细胞可能会导致离体的胰岛受损，外分泌细胞中的坏死可能主要是由于保存过程中已经存在的凋亡转化所致。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号