• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Islets >Histopaque provides optimal mouse islet purification kinetics: Comparison study with ficoll, iodixanol and dextran
【24h】

Histopaque provides optimal mouse islet purification kinetics: Comparison study with ficoll, iodixanol and dextran

机译：Histopaque提供最佳的小鼠胰岛纯化动力学：与ficoll，碘克沙醇和右旋糖酐的比较研究

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Islet transplantation has become a very promising treatment for type 1 diabetes. To facilitate further clinical improvements in this exciting field, rodent islets are used to evaluate new strategies and modifications. One method to purify islets is on a density gradient, although the optimal gradient component can be debated. N = 6 separate mouse islet isolations were used and the resulting islets were separated and purified on either a Ficoll, Histopaque, dextran or iodixanol gradient. Islets were assessed for recovery, viability, purity and in vitro functionality. Aliquots were transplanted into diabetic mice to assess in vivo functionality and survival. There was no difference in the number of islets recovered across groups nor in the size of recovered islets. Use of a Ficoll or Histopaque gradient led to the most pure and viable islets in comparison to dextran and iodixanol. Functionally, islets isolated on a Ficoll gradient had the highest glucose-stimulated insulin release in vitro while performing equally to Histopaque and dextran gradients in vivo. Using a Ficoll gradient, however, comes at a higher monetary cost. We recommend using a Histopaque gradient, which led to the isolation of viable and functional islets with a reduced cost as compared to a Ficoll gradient.
机译：胰岛移植已成为治疗1型糖尿病的非常有前途的方法。为了促进这一令人兴奋领域的进一步临床改进，啮齿类动物胰岛用于评估新的策略和修饰方法。纯化胰岛的一种方法是在密度梯度上进行，尽管可以讨论最佳梯度组分。使用N = 6个单独的小鼠胰岛分离物，并在Ficoll，Histopaque，右旋糖酐或碘克沙醇梯度上分离和纯化所得的胰岛。评估胰岛的回收率，生存力，纯度和体外功能。将等分试样移植到糖尿病小鼠中以评估体内功能和存活。各组之间回收的胰岛数量没有差异，回收的胰岛的大小也没有差异。与葡聚糖和碘克沙醇相比，使用Ficoll或Histopaque梯度可产生最纯净和可行的胰岛。从功能上讲，在Ficoll梯度上分离的胰岛在体外具有最高的葡萄糖刺激的胰岛素释放，而在体内的表现与Histopaque和葡聚糖梯度相同。但是，使用Ficoll梯度会带来更高的货币成本。我们建议使用Histopaque梯度，与Ficoll梯度相比，它可以降低可行和功能化的胰岛的分离成本。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号