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首页> 外文期刊>Glia >Analysis of IL-6/gp130 Family Receptor Expression Reveals That in Contrast to Astroglia, Microglia Lack the Oncostatin M Receptor and Functional Responses to Oncostatin M
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Analysis of IL-6/gp130 Family Receptor Expression Reveals That in Contrast to Astroglia, Microglia Lack the Oncostatin M Receptor and Functional Responses to Oncostatin M

机译：对IL-6 / gp130家族受体表达的分析揭示了与星形胶质，小胶质细胞相比，缺乏制瘤素M受体和对制瘤素M的功能性反应

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摘要

The interleukin (IL)-6/gp130 family of cytokines (e.g., IL-6, IL-11, leukemia inhibitory factor (LIF) and oncostatin M (OSM)) play important roles in the central nervous system (CNS) during neuroinflammation and neurodevelopment. However, little is known regarding the responses by astroglia and microglia to this family of cytokines. Here the expression of the IL-6/gp130 cytokine receptors and subsequent signal pathway activation was examined in murine astrocytes and microglia in vitro. Astrocytes had high levels of OSMR mRNA while lower levels of IL-6R, LIFR and IL-11R mRNAs were also present. In comparison, in microglia there was no detectable OSMR mRNA, higher levels of IL-6R mRNA and lower levels of the LIFR and IL-11R mRNAs. The OSMR protein was present in astrocytes but was undetectable in microglia. Conversely, the IL-6R protein was present in microglia but not detectable in astrocytes. In astrocytes but not microglia, phosphorylation of STAT1 and STAT3 occurred in response to OSM, whereas both microglia and astrocytes responded to hyper-IL-6 (IL-6 linked to the soluble IL-6 receptor). Finally, in both microglia and astrocytes, OSM failed to activate NFB or induce iNOS and nitrite production. We conclude: (1) notable differences exist in the expression of receptors utilized by the IL-6/gp130 family of cytokines in astrocytes and microglia, and (2) the findings provide a molecular basis for the differential response to OSM by astrocytes versus microglia and demonstrate a fundamental means for achieving cellular specificity in the response of these glial cells to this cytokine. GLIA 2015;63:132-141
机译：白细胞介素（IL）-6 / gp130家族的细胞因子（例如IL-6，IL-11，白血病抑制因子（LIF）和抑瘤素M（OSM））在神经炎症和中枢神经系统（CNS）中起重要作用神经发育。然而，关于星形胶质细胞和小胶质细胞对该细胞因子家族的反应知之甚少。在这里，在小鼠星形胶质细胞和小胶质细胞中体外检查了IL-6 / gp130细胞因子受体的表达和随后的信号通路激活。星形胶质细胞具有高水平的OSMR mRNA，而较低水平的IL-6R，LIFR和IL-11R mRNA也存在。相比之下，在小胶质细胞中没有可检测到的OSMR mRNA，较高水平的IL-6R mRNA和较低水平的LIFR和IL-11R mRNA。 OSMR蛋白存在于星形胶质细胞中，但在小胶质细胞中未检测到。相反，IL-6R蛋白存在于小胶质细胞中，但在星形胶质细胞中却无法检测到。在星形胶质细胞而非小胶质细胞中，STAT1和STAT3的磷酸化是对OSM的反应，而小胶质细胞和星形胶质细胞均对高IL-6（IL-6与可溶性IL-6受体相连）有反应。最后，在小胶质细胞和星形胶质细胞中，OSM均未能激活NFB或诱导iNOS和亚硝酸盐生成。我们得出的结论是：（1）星形胶质细胞和小胶质细胞中IL-6 / gp130细胞因子家族利用的受体表达存在显着差异，（2）研究结果为星形胶质细胞与小胶质细胞对OSM的差异反应提供了分子基础。并且证明了在这些神经胶质细胞对该细胞因子的反应中实现细胞特异性的基本手段。 GLIA 2015; 63：132-141

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