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首页> 外文期刊>FEMS Microbiology Ecology >Depth-related change in archaeal community structure in a freshwater lake sediment as determined with denaturing gradient gel electrophoresis of amplified 16S rRNA genes and reversely transcribed rRNA fragments
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Depth-related change in archaeal community structure in a freshwater lake sediment as determined with denaturing gradient gel electrophoresis of amplified 16S rRNA genes and reversely transcribed rRNA fragments

机译：通过变性梯度凝胶电泳确定扩增的16S rRNA基因和反向转录的rRNA片段，确定了淡水湖泊沉积物中古细菌群落结构的深度相关变化

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摘要

Vertical changes in archaeal community structure in mesophilic freshwater lake sediment were investigated using denaturing gradient gel electrophoresis of amplified 16S rRNA genes and reversely transcribed 16S rRNA fragments. Electrophoretic band pattern of arcbaeal Community did not drastically change with depth. The archaeal 16S rRNA-based denaturing gradient gel electrophoresis band pattern was very similar to the 16S rDNA-based pattern. These results indicate that archaeal community structure does not drastically change throughout the 0-20 cm in depth, and most archaeal populations in the sediment retain extractable rRNA. Phylogenetic analysis of seven sequences retrieved from both DNA- and RNA-based denaturing gradient gel electrophoresis revealed that these sequences were divided into four major lineages; the kingdom Crenarchaeota, the order Methanomicrobiales, the family Methanosaetaceae, and an uncultured cluster within Euryarchaeota. The order Methanomicrobiales and the family Methanosaetaceae were the dominant methane-producing archaea in this profundal freshwater lake sediment. Moreover, we found Euryarchaeota that are not related to methanogens, and non-thermophilic Crenarchaeota. This suggests that archaeal populations other than methanogens in low-temperature sediment have been previously underestimated. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
机译：使用变性的16S rRNA基因和反向转录的16S rRNA片段进行变性梯度凝胶电泳，研究了中温淡水湖泊沉积物中古细菌群落结构的垂直变化。弧菌群落的电泳谱带图谱并没有随深度而急剧变化。基于古细菌的16S rRNA变性梯度凝胶电泳条带模式与基于16S rDNA的模式非常相似。这些结果表明，古细菌群落结构在整个0-20厘米深度处不会发生剧烈变化，并且沉积物中的大多数古细菌种群保留了可提取的rRNA。从基于DNA和RNA的变性梯度凝胶电泳中检索到的七个序列的系统发生分析表明，这些序列被分为四个主要谱系。 Crenarchaeota王国，Methanomicrobiales目，Methanosaetaceae科以及Euryarchaeota内的一个未养殖集群。在这个深水淡水湖沉积物中，甲烷微菌纲和甲烷藻科是主要的产甲烷古菌。此外，我们发现与产甲烷菌和非嗜热性Crenarchaeota无关的Euryarchaeota。这表明低温沉积物中除产甲烷菌外的古细菌种群以前被低估了。 （C）2004年欧洲微生物学会联合会。由Elsevier B.V.发布。保留所有权利。

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