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首页> 外文期刊>FEMS Microbiology Ecology >PhyloChip hybridization uncovered an enormous bacterial diversity in the rhizosphere of different potato cultivars: Many common and few cultivar-dependent taxa
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PhyloChip hybridization uncovered an enormous bacterial diversity in the rhizosphere of different potato cultivars: Many common and few cultivar-dependent taxa

机译：PhyloChip杂交在不同马铃薯品种的根际中发现了巨大的细菌多样性：许多常见而很少有依赖品种的分类单元

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摘要

The phylogenetic composition of bacterial communities in the rhizosphere of three potato cultivars grown at two distant field sites was analysed. Ribosomal gene fragments amplified from total community DNA were hybridized to PhyloChips. A total of 2432 operational taxonomic units (OTUs) were detected by the PhyloChips, of which 65% were found in the rhizosphere of all cultivars at both field sites. From all detected OTUs, 9% revealed a cultivar-dependent abundance at the one or the other field site and 4% at both sites. Differential abundance on the three cultivars was mainly observed for OTUs belonging to the Pseudomonadales, Actinomycetales and Enterobacteriales. More than 40% of OTUs belonging to Bradyrhizobiales, Sphingomonadales, Burkholderiales, Rhodocyclales, Xanthomonadales and Actinomycetales differed significantly in their abundance between the sites. A sequence analysis of six 16S rRNA gene clone libraries corresponded well with the taxonomic community structure evidenced by the PhyloChip hybridization. Most ribotypes matched OTUs detected by the PhyloChip. Those OTUs that responded to the potato cultivar at both field sites might be of interest in view of cultivar-specific effects on bacterial biocontrol strains and pathogens.
机译：分析了在两个遥远的田地上种植的三个马铃薯品种的根际细菌群落的系统发育组成。从总群落DNA扩增的核糖体基因片段与PhyloChips杂交。 PhyloChips总共检测到2432个操作分类单位（OTU），其中在两个田间所有品种的根际中发现65％。在所有检测到的OTU中，有9％的人在一个或另一个田间站点发现了一个依赖品种的丰度，在两个站点中都显示了4％。主要观察到三个属的假单胞菌属，放线菌属和肠杆菌属的OTUs的丰度差异。地点之间，有超过40％的OTU属于Bradyrhizobiales，Sphingomonadales，Burkholderiales，Rhodocyclales，Xanthomonadales和Actinomycetales的OTU差异很大。六个16S rRNA基因克隆文库的序列分析与PhyloChip杂交所证明的分类学群落结构非常吻合。大多数核糖型均与PhyloChip检测到的OTU相匹配。考虑到对细菌生物控制菌株和病原体的特定品种效应，那些在两个田间地点都对马铃薯品种产生响应的OTU可能会引起人们的兴趣。

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