• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>FEMS Microbiology Letters >Quantification of bacterial mRNA involved in degradation of 1,2,4-trichlorobenzene by Pseudomonas sp. strain P51 from liquid culture and from river sediment by reverse transcriptase PCR (RT/PCR)
【24h】

Quantification of bacterial mRNA involved in degradation of 1,2,4-trichlorobenzene by Pseudomonas sp. strain P51 from liquid culture and from river sediment by reverse transcriptase PCR (RT/PCR)

机译：定量细菌假单胞菌sp降解1,2,4-三氯苯所涉及的mRNA。逆转录酶PCR（RT / PCR）从液体培养和河流沉积物中分离出P51菌株

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Competitive reverse transcriptase polymerase chain reaction (RT/PCR) was used to quantify the mRNA of the tcbC gene of Pseudomonas sp. strain P51. The tcbC gene encodes the enzyme chlorocatechol-1,2-dioxygenase involved in 1,2,4-trichlorobenzene (TCB) degradation. The mRNA content per cell was monitored in a batch culture growing on 1,2,4-TCB. No mRNA could be detected in the first 2 days of the lag phase. mRNA production became maximal with 20 molecules per cell in the early exponential growth phase but then decreased to less than 10 molecules per cell. When TCB was depleted and the cells entered the stationary phase, the mRNA content decreased slowly below the detection limit within 4 days. In order to compare detection of tcbC mRNA in pure culture and in river sediment, cells of strain P51 pregrown on TCB were added to sediment and RNAs extracted. In sediment samples containing 5x10(8) cells per gram the tcbC mRNA was quantifiable by RT/PCR. The mRNA recovery was about 3% as compared to the inoculum. The detection limit of the RT/PCR method was about 10(7) mRNA molecules per gram sediment or 10(6) copies per mi culture medium which corresponded in our case to 10(5) molecules per reaction vial. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved. [References: 14]
机译：使用竞争性逆转录酶聚合酶链反应（RT / PCR）定量假单胞菌sp。tcbC基因的mRNA。 P51株。 tcbC基因编码参与1,2,4-三氯苯（TCB）降解的氯邻苯二酚1,2-双加氧酶。在生长于1,2,4-TCB的分批培养物中监测每个细胞的mRNA含量。在滞后阶段的前2天未检测到mRNA。在早期的指数生长期，mRNA的产量最高，每个细胞有20个分子，但随后下降到每个细胞少于10个分子。当TCB耗尽并且细胞进入固定相时，mRNA含量在4天内缓慢下降到检测极限以下。为了比较在纯培养物中和河底沉积物中tcbC mRNA的检测，将在TCB上预生长的P51菌株细胞添加到沉积物中并提取RNA。在每克含5x10（8）个细胞的沉积物样品中，tcbC mRNA可通过RT / PCR定量。与接种物相比，mRNA的回收率约为3％。 RT / PCR方法的检测极限是每克沉淀物约10（7）个mRNA分子或每mi培养基10（6）个拷贝，在我们的情况下相当于每个反应瓶10（5）个分子。 （C）1998年欧洲微生物学会联合会。由Elsevier Science B.V.保留所有权利。 [参考：14]

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号