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首页> 外文期刊>Food and Bioproducts Processing. Transactions of the Institution of Chemical Engineers, Part C >An alternative method for the decoloration of epsilon-poly-L-lysine eluate by macroporous resin in the separation and purification of epsilon-poly-L-lysine from fermentation broth
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An alternative method for the decoloration of epsilon-poly-L-lysine eluate by macroporous resin in the separation and purification of epsilon-poly-L-lysine from fermentation broth

机译：从发酵液中分离和纯化ε-聚-L-赖氨酸的大孔树脂对ε-聚-L-赖氨酸洗脱液脱色的另一种方法

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摘要

To develop an efficient strategy for the decoloration of e-poly-L-lysine (epsilon-PL) produced by microbial fermentation with Streptomyces, macroporous resin was firstly investigated on the removal of pigments in e-PL ion exchange eluate here. SX-8 resin was found to be the best through static and dynamic comparison tests, which adsorbed the most pigments and the least epsilon-PL. Under the optimized dynamic adsorption conditions, i.e. pH 7.0, 30 degrees C, 1 BV/h uploading rate, 7 g/L epsilon-PL concentration, SX-8 resin was employed to the treatment of 100 mL (10 BV) epsilon-PL ion exchange eluate, and the resulting decoloration ratio was 83.9% with a recovery ratio of epsilon-PL 97.3%. Further spectrum analysis showed that pigments with absorption wavelength between 300 and 900 nm were effectively removed after treatment by SX-8 resin. The results presented reveal that SX-8 resin is promising for scale-up epsilon-PL extraction and purification from fermentation broth. (C) 2014 The Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.
机译：为了开发一种有效的策略，通过链霉菌微生物发酵生产的e-聚-L-赖氨酸（epsilon-PL）脱色，首先在这里研究了大孔树脂在e-PL离子交换洗脱液中去除色素的方法。通过静态和动态对比测试，发现SX-8树脂是最好的，它吸附了最多的颜料和最少的epsilon-PL。在优化的动态吸附条件下，即pH 7.0、30摄氏度，1 BV / h上载速率，7 g / L epsilon-PL浓度，使用SX-8树脂处理100 mL（10 BV）epsilon-PL离子交换洗脱液，所得的脱色率为83.9％，ε-PL的回收率为97.3％。进一步的光谱分析表明，经SX-8树脂处理后，吸收波长在300至900 nm之间的颜料被有效去除。呈现的结果表明，SX-8树脂有望从发酵液中按比例放大epsilon-PL提取和纯化。 （C）2014化学工程师学会。由Elsevier B.V.发布。保留所有权利。

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