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首页> 外文期刊>Food and Chemical Toxicology: An International Journal Published for the British Industrial Biological Research >Myricetin affords protection against peroxynitrite-mediated DNA damage and hydroxyl radical formation.
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Myricetin affords protection against peroxynitrite-mediated DNA damage and hydroxyl radical formation.

机译：杨梅素提供针对过氧亚硝酸盐介导的DNA损伤和羟基自由基形成的保护作用。

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摘要

Peroxynitrite has been extensively implicated in the pathogenesis of various forms of neurodegenerative disorders via its cytotoxic effects, this study was undertaken to investigate whether the neuroprotective effect of myricetin is associated with inhibition of peroxynitrite-mediated DNA damage, a critical event leading to peroxynitrite elicited cytotoxicity. We observed that peroxynitrite can cause DNA damage in varphiX-174 plasmid DNA and rat primary astrocytes. The presence of myricetin at physiological concentration was found to significantly inhibit DNA strand breakage induced by both peroxynitrite and its generator 3-morpholinosydnonimine (SIN-1). Moreover, the consumption of oxygen caused by SIN-1 was found to be decreased in the presence of myricetin, indicating that myricetin might affect the auto-oxidation of SIN-1. Furthermore, EPR spectroscopy demonstrated that the formation of DMPO-hydroxyl radical adduct (DMPO-OH) from peroxynitrite, and that myricetin inhibited the adduct signal in a concentration-dependent manner. Taken together, these results demonstrate for the first time that myricetin can inhibit peroxynitrite-mediated DNA damage and hydroxyl radical formation.
机译：过氧亚硝酸盐通过其细胞毒性作用已广泛参与各种形式的神经退行性疾病的发病机理，本研究旨在研究杨梅素的神经保护作用是否与抑制过氧亚硝酸盐介导的DNA损伤有关，这是导致过氧亚硝酸盐引起细胞毒性的关键事件。 。我们观察到过氧亚硝酸盐可导致varphiX-174质粒DNA和大鼠原代星形胶质细胞中的DNA损伤。发现在生理浓度下杨梅素的存在可显着抑制过氧亚硝酸盐及其产生剂3-吗啉代亚砜亚胺（SIN-1）诱导的DNA链断裂。此外，发现在杨梅素的存在下由SIN-1引起的氧气消耗减少了，这表明杨梅素可能会影响SIN-1的自氧化。此外，EPR光谱表明过氧亚硝酸盐形成了DMPO-羟基自由基加合物（DMPO-OH），而杨梅素以浓度依赖性方式抑制了加合物信号。综上所述，这些结果首次证明了杨梅素可以抑制过氧亚硝酸盐介导的DNA损伤和羟基自由基的形成。

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