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首页> 外文期刊>Food Control >High throughput cellular biosensor for the ultra-sensitive, ultra-rapid detection of aflatoxin M1.
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High throughput cellular biosensor for the ultra-sensitive, ultra-rapid detection of aflatoxin M1.

机译：高通量细胞生物传感器，用于黄曲霉毒素M1的超灵敏，超快速检测。

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The aim of this study was the development of a rapid novel biosensor system based on the Bioelectric Recognition Assay (BERA) for the detection of aflatoxin M1 (AFM1). Membrane-engineering was achieved by electroinsertion of AFM1-homologous antibodies into the membrane of the cells. The sensor measured the electric response of cultured membrane-engineered fibroblast cells suspended in an alginate gel matrix due to the change of their membrane potential after the interaction of the analyte molecules with the antibodies. The BERA-based sensor was able to detect AFM1 rapidly (3 min) at very low concentrations (5 pg/ml = 5 ppt), thus demonstrating a higher sensitivity than most currently available biosensor-based methods. In addition, the assay was quite selective against other aflatoxins, such as aflatoxin B1 and ochratoxin A. Furthermore, the assay system allowed for high throughput AFM1 analysis (160 individual tests/h), due to its multiple cell-electrode interface array. Although further research is required for the optimization of the assay, the elimination of possible matrix effects and its validation by assaying actual food samples, this novel biosensor offers new perspectives for ultra-rapid, ultra-sensitive and low-cost monitoring of mycotoxins in food commodities. All rights reserved, Elsevier.
机译：这项研究的目的是开发一种基于生物电识别分析（BERA）的快速新型生物传感器系统，用于检测黄曲霉毒素M1（AFM1）。通过将AFM1同源抗体电插入细胞膜中来实现膜工程化。该传感器测量了在分析物分子与抗体相互作用后由于膜电位的变化而悬浮在藻酸盐凝胶基质中的培养的膜工程化成纤维细胞的电响应。基于BERA的传感器能​​够以极低的浓度（5 pg / ml = 5 ppt）快速（3分钟）检测AFM1，因此比大多数当前基于生物传感器的方法具有更高的灵敏度。此外，该测定法对其他黄曲霉毒素（如黄曲霉毒素B1和曲霉毒素A）具有很高的选择性。此外，由于其多细胞-电极界面阵列，该测定系统可进行高通量AFM1分析（160次独立检测/小时）。尽管需要进行进一步的研究以优化测定方法，消除可能的基质效应并通过测定实际食品样品来验证其有效性，但这种新型生物传感器为食品中真菌毒素的超快速，超灵敏和低成本监测提供了新的见解商品。保留所有权利，Elsevier。

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