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首页> 外文期刊>Folia histochemica et cytobiologica >Immunolocalization of estrogen receptor beta in the epididymis of mature and immature pigs.
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Immunolocalization of estrogen receptor beta in the epididymis of mature and immature pigs.

机译：雌激素受体β在成熟和未成熟猪的附睾中的免疫定位。

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摘要

A growing body of evidence suggests a role of estrogens in the male reproduction via their specific estrogen receptors (ERalpha/ERbeta). Estrogen receptor distribution along the genital tract tissues has been described in different species, but it is unknown in the pig. Therefore, the aim of the present study was to localize ERbeta in the epididymis of mature and immature pigs (aged 2 and 18 months, respectively). Immunohistochemistry was carried out on paraffin-embedded tissues using a mouse anti-human monoclonal IgG against ERbeta as the primary antibody, and a goat anti-mouse biotinylated IgG as the secondary antibody. Avidin-biotin-peroxidase complex was then applied followed by diaminobenzidine. In immature pigs, the epithelial cells from the caput, corpus and cauda epididymis showed no or very weak immunoreactivity for ERbeta, whereas they were all strongly immmunoreactive in mature pigs. A various intensity of immunostaining from weak to strong in the smooth muscle cells as well as in the connective tissue cells were detected in the epididymis of both, young and adult pigs. This is the first report on the cellular localization of ERbeta protein in porcine epidydimis. The present study demonstrated that (1) irrespectively of the epididymal region, the epithelial cells of caput, corpus and cauda epididymis of mature pigs revealed a strong immunoreactivity for ERbeta, and (2) ERbeta expression in the epididymal epithelium is regulated by puberty. Finally, although the biological activity of ERbeta has not yet been established, the results of the present study suggest its involvement in estrogen modulation of pig epididymal function.
机译：越来越多的证据表明，雌激素通过其特定的雌激素受体（ERalpha / ERbeta）在男性生殖中发挥作用。沿生殖道组织的雌激素受体分布已在不同物种中描述，但在猪中未知。因此，本研究的目的是将ERbeta定位在成熟和未成熟猪（分别为2个月和18个月大）的附睾中。使用针对ERbeta的小鼠抗人单克隆IgG作为第一抗体，对山羊抗小鼠生物素化IgG作为第二抗体，在石蜡包埋的组织上进行了免疫组织化学。然后施加抗生物素蛋白-生物素-过氧化物酶复合物，然后施加二氨基联苯胺。在未成熟的猪中，来自于鸭ut，体和附睾马尾的上皮细胞对ERbeta的免疫反应性很小或很弱，而在成熟猪中它们的免疫反应性很强。在幼猪和成年猪的附睾中均检测到平滑肌细胞以及结缔组织细胞中从弱到强的各种免疫染色强度。这是关于ERbeta蛋白在猪附睾中细胞定位的首次报道。本研究表明（1）不论在附睾区域，成熟猪的头、,体和马尾附睾的上皮细胞均显示出对ERbeta的强免疫反应性;（2）ERbeta在附睾上皮中的表达受到青春期的调节。最后，尽管尚未确定ERbeta的生物学活性，但本研究结果表明其参与猪附睾功能的雌激素调节。

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