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首页> 外文期刊>Folia histochemica et cytobiologica >Investigations of hippocampal astrocytes in lipopolysaccharide-preconditioned rats in the pilocarpine model of epilepsy
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Investigations of hippocampal astrocytes in lipopolysaccharide-preconditioned rats in the pilocarpine model of epilepsy

机译：毛果芸香碱癫痫模型中脂多糖预处理大鼠海马星形胶质细胞的研究

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The present paper is the first work to determine the effect of lipopolysaccharide (LPS) in the pilocarpine model of epilepsy on the morphology of rat hippocampal astrocytes in vivo. The study involved adult male Wistar rats, which 72 hours prior to administration of pilocarpine hydrochloride (PILO) were intraperitoneally (ip) preconditioned with LPS at a dose of 0.5 mg/kg b.w. The control animals were administered (ip) saline or LPS alone. The astrocytes in the control animals displayed characteristic stellate morphology. Examinations of the astrocytes were performed on days one, three and 21 of the pilocarpine model of epilepsy (i.e. in the acute, silent and chronic periods). The astrocytes of the CA1 and CA3 pyramidal layers of the hippocampus were observed and analyzed at the structural and ultrastructural levels. It was demonstrated that on days one and three, glial cells from both the nonpreconditioned and the LPS-preconditioned animals displayed similar reactive changes, manifesting themselves as swelling of cell bodies, glial processes, and astrocytosis. Moreover, reduction in cell organelles aggregated at one pole and the presence of vacuoles were observed. The most pronounced astrogliosis and cell swelling occurred on day 21. We conclude that LPS has no effect on the morphology of astrocytes in the pilocarpine model of epilepsy, unlike the results obtained by other authors in vitro.
机译：本文是确定脂蛋白（LPS）在癫痫的毛状结肠模型中对大鼠海马星形胶质细胞形态的影响的第一项工作。该研究涉及成年雄性Wistar大鼠，在给予盐酸匹洛卡品（PILO）之前72小时，腹膜内（ip）用LPS进行了0.5 mg / kg b.w的预处理。对照动物单独给予（ip）盐水或LPS。对照动物中的星形胶质细胞显示出特征性的星状形态。在毛果芸香碱癫痫模型的第一天，第三天和第二十一天（即在急性期，沉默期和慢性期）进行星形胶质细胞的检查。在结构和超结构水平上观察和分析海马CA1和CA3锥体层的星形胶质细胞。结果表明，在第1天和第3天，未预处理动物和LPS预处理动物的神经胶质细胞表现出相似的反应性变化，表现为细胞体肿胀，神经胶质过程和星形细胞增多。此外，观察到在一个极点聚集的细胞器的减少和液泡的存在。最明显的星形胶质细胞增生和细胞肿胀发生在第21天。我们得出结论，与其他作者体外获得的结果不同，LPS对匹罗卡品癫痫模型中的星形胶质细胞形态没有影响。

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