• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Forensic science international >Application of plant DNA markers in forensic botany: genetic comparison of Quercus evidence leaves to crime scene trees using microsatellites.
【24h】

Application of plant DNA markers in forensic botany: genetic comparison of Quercus evidence leaves to crime scene trees using microsatellites.

机译：植物DNA标记在法医植物学中的应用：使用微卫星将栎属证据叶与犯罪现场树进行遗传比较。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

As highly polymorphic DNA markers become increasingly available for a wide range of plant and animal species, there will be increasing opportunities for applications to forensic investigations. To date, however, relatively few studies have reported using DNA profiles of non-human species to place suspects at or near crime scenes. Here we describe an investigation of a double homicide of a female and her near-term fetus. Leaf material taken from a suspect's vehicle was identified to be that of sand live oak, Quercus geminata, the same tree species that occurred near a shallow grave where the victims were found. Quercus-specific DNA microsatellites were used to genotype both dried and fresh material from trees located near the burial site and from the material taken from the suspect's car. Samples from the local population of Q. geminata were also collected and genotyped in order to demonstrate that genetic variation at four microsatellite loci was sufficient to assign leaves to an individual tree with high statistical certainty. The cumulative average probability of identity for these four loci was 2.06x10(-6). DNA was successfully obtained from the dried leaf material although PCR amplification was more difficult than amplification of DNA from fresh leaves. The DNA profiles of the dried leaves from the suspect's car did not match those of the trees near the crime scene. Although this investigation did not provide evidence that could be used against the suspect, it does demonstrate the potential for plant microsatellite markers providing physical evidence that links plant materials to live plants at or near crime scenes.
机译：随着高度多态的DNA标记越来越广泛地用于各种动植物物种，法医研究的应用机会将越来越多。然而，迄今为止，很少有研究报道使用非人类物种的DNA谱图将犯罪嫌疑人放置在犯罪现场或附近。在这里，我们描述了对女性及其近期胎儿的双重杀人案的调查。经鉴定，从犯罪嫌疑人的车辆中提取的叶片材料是橡树栎栎（Quercus geminata）的橡树栎，与发现受害者的浅坟墓附近的树木相同。 Quercus特有的DNA微卫星被用来对来自埋葬地点附近的树木以及从犯罪嫌疑人的汽车上获取的干燥和新鲜材料进行基因分型。为了证明在四个微卫星基因座上的遗传变异足以将叶子分配给具有较高统计确定性的单棵树，还对来自双子甘蓝当地种群的样品进行了收集并进行了基因分型。这四个基因座的同一性的累积平均概率为2.06x10（-6）。尽管从鲜叶中扩增DNA比PCR扩增更困难，但从干燥的叶片材料中成功获得了DNA。犯罪嫌疑人的汽车上的干树叶的DNA轮廓与犯罪现场附近的树木的DNA轮廓不匹配。尽管此调查并未提供可用于对嫌疑人的证据，但确实证明了植物微卫星标记物提供了将植物材料与犯罪现场或附近的活植物联系起来的物理证据的潜力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号