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Lucilia silvarum Meigen, 1826 (Diptera: Calliphoridae)-A new species of interest for forensic entomology in Europe

机译:Lucilia silvarum Meigen,1826年(双翅目:Calliphoridae)-欧洲法医昆虫学的一个新物种

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摘要

In Europe, the blowfly genus Lucilia is represented in Forensic Entomology mainly by the species L. ampullacea, L. caesar, L. illustris and L. sericata. In the US, Lucilia silvarum is rarely recorded as a carrion breeding species but usually as a more or less exclusive parasite of frogs and toads. We present three forensic cases from different European countries reporting, for the first time, L. silvarum on human bodies that were found close to lakes, wetlands, or riversides. To use this species for post-mortem interval estimations, thermal development data is needed. The first step is accurate identification by morphological and molecular means. Therefore, we analysed a 611. bp part of the mitochondrial COI region for 23 specimens of L. silvarum from 9 different geographical regions, all of which give the same haplotype. Differences within the haplotype varied by up to 0.2%. Comparison between the haplotype found and those published on GenBank showed up to 1.2% variance. Moreover, we present an updated key for the morphological identification of the third larval instars of European Lucilia spp. of forensic importance, adding not only L. silvarum, but also L. cuprina which was recorded in Europe for the first time about 20 years ago.
机译:在欧洲,在法医昆虫学中,蝇fly属的卢西莉亚(Lucilia)主要由壶腹乳杆菌(L. ampullacea),凯撒乳杆菌(L. caesar),鸢尾乳杆菌(L. illustris)和丝纹乳杆菌(L. sericata)代表。在美国,极少有Lucilia silvarum被记录为腐肉繁殖物种,但通常或多或少是青蛙和蟾蜍的寄生虫。我们介绍了来自不同欧洲国家的三宗法医案例,这是首次报告了在湖,湿地或河边附近发现的人体上的L. silvarum。为了将该物种用于验尸间隔估计,需要热发育数据。第一步是通过形态学和分子手段进行准确鉴定。因此,我们分析了来自9个不同地理区域的23份L. silvarum线粒体COI区域的611.bp片段,它们均具有相同的单倍型。单倍型内的差异最多变化0.2%。发现的单倍型与GenBank上发表的单倍型之间的比较显示出高达1.2%的差异。此外,我们提出了欧洲Lucilia spp的第三龄幼虫形态鉴定的更新密钥。具有法医学意义,不仅增加了L. silvarum,而且增加了大约20年前在欧洲首次记录的L. cuprina。

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