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首页> 外文期刊>Forensic science international >Prescription and illicit psychoactive drugs in oral fluid-LC-MS/MS method development and analysis of samples from Brazilian drivers
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Prescription and illicit psychoactive drugs in oral fluid-LC-MS/MS method development and analysis of samples from Brazilian drivers

机译：口服液中的处方药和违禁精神药物-LC-MS / MS方法开发和巴西驾驶员样品分析

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This study is part of a larger project designed to investigate the prevalence of psychoactive drug (PAD) use among Brazilian drivers. In this paper we describe the development and validation of an analytical method to analyze 32 prescription and illicit PADs (amphetamines, benzodiazepines, cocaine, cannabis, opioids, ketamine and m-CPP) and metabolites in oral fluid samples collected with a Quantisal? device. Samples were extracted with ethyl acetate:hexane and analyzed by LC-MS/MS. Instrumental LOD ranged from 0.26 to 0.65. ng/mL. Mean procedural recoveries at 1.3. ng/mL (LLOQ) ranged from 50% to 120% for 24 compounds. Recoveries were concentration independent, with the exception of femproporex, heroin and ecgonine methyl-ester (EME) for which the recovery decreased significantly at higher levels (13 and 52. ng/mL). RSD was <20% for all compounds at all spiking levels. Ion suppression due to the matrix was <20% for most compounds, and higher than 60% for EME and diethylpropion. Analysis was performed against a in-matrix standard curve. About 10% of the 2235 oral fluid samples collected from drivers on Brazilian Federal highways were positive (≥LOD) for at least one analyte investigated. Alone or in combination with other drugs, cocaine/metabolites were the analytes most detected in the samples (129; 5.8%), followed by amphetamines/metabolite (69; 3.1%), benzodiazepines (28; 1.2%), cannabinoids (23; 1.1%) and opioids (8; 0.4%). Detection of at least two PADs from different classes accounted for 9.3% of the 236 positive samples. Cocaine was found at higher levels in the samples (up to 1165. ng/mL). Preventive measures aimed at reducing the use of PADs by drivers in Brazil will certainly contribute to decrease the country's highway death rates.
机译：这项研究是一个较大项目的一部分，该项目旨在调查巴西驾驶员中使用精神活性药物（PAD）的患病率。在本文中，我们描述了一种分析方法的开发和验证，该方法可用于分析使用Quantisal？收集的口腔液样品中的32种处方和非法PAD（苯丙胺，苯二氮卓，可卡因，大麻，阿片类药物，氯胺酮和m-CPP）和代谢物。设备。用乙酸乙酯：己烷萃取样品，并通过LC-MS / MS分析。仪器的LOD范围从0.26到0.65。 ng / mL。平均程序恢复率为1.3。 24种化合物的ng / mL（LLOQ）为50％至120％。回收率与浓度无关，除femproporex，海洛因和芽子碱甲酯（EME）外，其回收率在较高水平（13和52. ng / mL）时明显下降。在所有加标水平下，所有化合物的RSD <20％。对于大多数化合物，由于基质引起的离子抑制率<20％，对于EME和二乙基丙酸则高于60％。针对矩阵内标准曲线进行分析。在巴西联邦高速公路上从驾驶员那里收集的2235口液样本中，至少有一种被分析物为阳性（≥LOD），约占10％。可卡因/代谢产物是单独或与其他药物合用的样品，是样品中检测到最多的分析物（129; 5.8％），其次是苯丙胺/代谢产物（69; 3.1％），苯二氮卓类药物（28; 1.2％），大麻素（23; 69）。 1.1％）和阿片类药物（8; 0.4％）。在236个阳性样本中，检测到至少两个来自不同类别的PAD占9.3％。在样品中发现可卡因含量较高（高达1165. ng / mL）。旨在减少巴西驾驶员使用PAD的预防措施肯定会有助于降低该国的公路死亡率。

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