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首页> 外文期刊>Forest Pathology >Phenotypic characterization of cryptic Diplodia species by MALDI-TOF MS and the bias of mycelium age
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Phenotypic characterization of cryptic Diplodia species by MALDI-TOF MS and the bias of mycelium age

机译：MALDI-TOF MS对隐孢子虫种的表型鉴定和菌丝体年龄的偏差

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Detection and identification of fungal cryptic species has been facilitated by DNA sequencing. However, the examination of some phenotypic traits is fundamental for the confirmation of genetic results. MALDI-TOF mass spectrometry has shown remarkable reliability in the recognition of species-specific phenotypic markers and has already been tested in several species of fungi. However, even though there is no direct evidence, the age of the mycelium appears to influence the composition of the metabolites detected. In this study, we demonstrate that MALDI-TOF is a reliable technique to identify suitable metabolites to distinguish three monophyletic species of Diplodia (D.pinea, D.seriata and D.scrobiculata), thus supporting recent DNA results. Nevertheless, different collections of samples over a period of 3months after inoculation also revealed that the MALDI-TOF spectra are highly dependent on mycelial age. More importantly, the species-specific markers emerge only after 1month of mycelial growth. The methodological and biological implications of these findings are discussed.RI Dapporto, Leonardo/E-5752-2010
机译：DNA测序促进了真菌隐性物种的检测和鉴定。但是，对某些表型性状的检查对于确认遗传结果至关重要。 MALDI-TOF质谱法在识别物种特异性表型标记物方面显示出显着的可靠性，并且已经在几种真菌中进行了测试。但是，即使没有直接的证据，菌丝体的年龄似乎也会影响所检测到的代谢物的组成。在这项研究中，我们证明了MALDI-TOF是一种可靠的技术，可用于识别合适的代谢物以区分双翅类的三种单系物种（D.pinea，D.seriata和D.scrobiculata），从而支持最新的DNA结果。尽管如此，接种后3个月内样品的不同收集也显示MALDI-TOF光谱高度依赖于菌丝体年龄。更重要的是，物种特异性标记仅在菌丝体生长1个月后出现。讨论了这些发现的方法学和生物学意义.RI Dapporto，Leonardo / E-5752-2010

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