Species-specific PCR for rapid identification of Ganoderma philippii and Ganoderma mastoporum from Acacia mangium and Eucalyptus pellita plantations in Indonesia

Yuskianti V.; Glen M.; Puspitasari D.; Francis A.; Rimbawanto A.; Gafur A.; Indrayadi H.; Mohammed C. L.

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Species-specific PCR for rapid identification of Ganoderma philippii and Ganoderma mastoporum from Acacia mangium and Eucalyptus pellita plantations in Indonesia

机译：物种特异性PCR用于快速鉴定印度尼西亚马占相思和桉树人工林中的菲律宾灵芝和马氏灵芝

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DNA sequence variation in the rDNA internal transcribed spacers (ITS) was exploited to design species-specific primers for Ganoderma philippii and Ganoderma mastoporum, two fungi associated with red root rot in Acacia mangium and other perennial crops. Specificity was verified against five other Ganoderma species, 34 other basidiomycete species and two ascomycete species. The PCR tests assisted in the identification of 822 fungal isolates obtained from A.mangium and Eucalyptus pellita plantations in Indonesia. These tests have provided a high level of confidence in the identification of the major root rot pathogens, information that can be used to support the development of management options for their control in A.mangium plantations.

机译：利用rDNA内部转录间隔区（ITS）中的DNA序列变异设计针对菲律宾灵芝和灵芝的物种特异性引物，这两种真菌与马占相思和其他多年生作物的红根腐烂有关。验证了针对其他五个灵芝物种，34个其他担子菌物种和两个子囊菌物种的特异性。 PCR测试有助于鉴定从印度尼西亚的A.mangium和Eucalyptus pellita人工林获得的822种真菌分离物。这些测试为主要根腐病病原体的鉴定提供了高度的信心，这些信息可用于支持在芒果种植园中控制其发展的管理选择。

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《Forest Pathology》 |2014年第6期|共9页
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Yuskianti V.; Glen M.; Puspitasari D.; Francis A.; Rimbawanto A.; Gafur A.; Indrayadi H.; Mohammed C. L.;
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1. Species-specific PCR for rapid identification of Ganoderma philippii and Ganoderma mastoporum from Acacia mangium and Eucalyptus pellita plantations in Indonesia [J] . Yuskianti V., Glen M., Puspitasari D., Forest Pathology . 2014,第6期

机译：物种特异性PCR用于快速鉴定印度尼西亚马占相思和桉树人工林中的菲律宾灵芝和马氏灵芝
2. Sexuality and mating types of Ganoderma philippii, Ganoderma mastoporum and Ganoderma australe, three basidiomycete fungi with contrasting ecological roles in south-east Asian pulpwood plantations [J] . Page D. E., Glen M., Puspitasari D., Australasian Plant Pathology . 2018,第1期

机译：灵芝菲利普利，灵芝甲基葡萄球菌和灵芝的性感和交配类型，三种盆地真菌与东南亚副工程组织对比生态作用
3. Disease progression in plantations of Acacia mangium affected by red root rot (Ganoderma philippii) [J] . Francis A., Beadle C., Puspitasari D., Forest Pathology . 2014,第6期

机译：马占相思人工林受红根腐病的病害进展
4. Active alkali charge effect on kraft pulping process of acacia mangium and eucalyptus pellita [C] . Vebria Ardina, Bambang Irawan, Danawati Hari Prajitno, International Conference on Science and Applied Science . 2018

机译：Acacia Mangium牛肝制浆过程的活性碱电荷效应，桉树Pellita
5. Genome-based species-specific primers for rapid identification of six species of Lactobacillus acidophilus group using multiplex PCR [O] . Inhwan You, Eun Bae Kim 2020

机译：基于基于基因组的物种特异性引物用于快速鉴定使用多重PCR的六种乳杆菌嗜酸乳杆菌基团
6. A single dominant Ganoderma species is responsible for root rot of Acacia mangium and Eucalyptus in Sumatra [O] . M Pa Coetzee A, B D Wingfield A, G D Golani B, 2012

机译：单一的显性灵芝物种是苏门答腊马占相思和桉树根腐病的原因

Species-specific PCR for rapid identification of Ganoderma philippii and Ganoderma mastoporum from Acacia mangium and Eucalyptus pellita plantations in Indonesia

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