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首页> 外文期刊>Forest Pathology >Molecular characterization and functional analysis of a calponin gene from the pinewood nematode
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Molecular characterization and functional analysis of a calponin gene from the pinewood nematode

机译：松材线虫中钙蛋白基因的分子表征和功能分析

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摘要

In this study, a cDNA sequence corresponding to the Bursaphelenchus xylophilus unc-87 homolog gene (Bx-unc-87) was identified in B. xylophilus transcriptome and characterized. This cDNA has an 1152-bp-long open-reading frame that is putatively translated into a 383 amino acids peptide. The deduced protein (Bx-UNC-87) contains highly conserved regions of the calponin-like proteins and has high sequence identity to the calponin proteins of other nematodes. In Caenorhabditis elegans, the calponin protein encoded by gene unc-87 is required to maintain the structure of myofilaments in muscle cells of the body wall. The silencing of Bx-unc-87 gene was performed by RNAi, soaking the nematodes in a solution containing dsRNA of this gene. In addition to a no dsRNA control, a non-homologous dsRNA control was also included. The Bx-unc-87 transcription was downregulated in nematodes treated with Bx-unc-87 dsRNA in comparison with the non-dsRNA-treated nematodes. dsRNA-treated nematodes revealed some paralysis and uncoordinated movement in contrast to the regular and sinusoidal movement of the non-treated nematodes. Furthermore, reproduction was reduced in dsRNA-treated nematodes. These results suggest that this protein has a function similar to that of the C. elegans homolog.
机译：在这项研究中，在木糖双歧杆菌转录组中鉴定了对应于木糖双歧杆菌unc-87同源基因（Bx-unc-87）的cDNA序列。该cDNA具有1152 bp长的开放阅读框，该框架可能被翻译成383个氨基酸的肽。推导的蛋白质（Bx-UNC-87）包含钙蛋白酶样蛋白的高度保守区域，并且与其他线虫的钙蛋白蛋白具有高度的序列同一性。在秀丽隐杆线虫中，需要由unc-87基因编码的钙蛋白酶蛋白来维持体壁肌肉细胞中的肌丝结构。 Bx-unc-87基因的沉默是通过RNAi进行的，将线虫浸泡在含有该基因dsRNA的溶液中。除无dsRNA对照外，还包括非同源dsRNA对照。与未用dsRNA处理的线虫相比，用Bx-unc-87 dsRNA处理的线虫中的Bx-unc-87转录被下调。 dsRNA处理的线虫与未处理的线虫的规则和正弦波运动相比，显示出一些麻痹和不协调的运动。此外，在dsRNA处理的线虫中繁殖减少。这些结果表明该蛋白质具有类似于秀丽隐杆线虫同源物的功能。

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