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首页> 外文会议>Symposium of the British Crop Protection Council >Molecular identification and pathway analysis of the introduced pinewood nematode Bursaphelenchus xylophilus
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Molecular identification and pathway analysis of the introduced pinewood nematode Bursaphelenchus xylophilus

机译：引进的Pinewood Nematode Bursaphelenchus Xylophilus的分子鉴定和途径分析

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The pinewood nematode (PWN) {Bursapheletichus xylophilus) is native to North America. In the past this invasive species has been spread by means of timber trade and package wood to several East Asian countries and, more recently, to Portugal (Evans etal., 1996; Mota et al., 1999). PWN is pathogenic to many pine species in its new areas of distribution, causing economic loss due to pine wilt disease. The identification of PWN and its differentiation from other species of Bursaphelenchus has been facilitated by application of the rDNA ITS-RFLP technique (Braasch et al, 1999). In spite of EU quarantine regulations, living PWNs were found in 47 samples of conifer wood imported to EU countries during 2000 to 2004. In order to trace the origin of the PWN populations introduced to Portugal, Random Amplified Polymorphic DNA-PCR (RAPD-PCR) was performed to determine genetic relationships among PWN populations from different countries.
机译：Pinewood Nematode（Pwn）{Bursapheletchetchus Xylophilus）是原产于北美。在过去，这种侵入性物种已经通过木材贸易和包装木头传播到几个东亚国家，而且最近到葡萄牙（埃文斯·埃拉尔。，1996; Mota等，1999）。 PWN在其新的分布领域是许多杉木物种的致病性，导致由于松枯萎病导致经济损失。通过施用RDNA其-RFLP技术（Braasch等人，1999），促进了PWN的鉴定及其与其他种类物种的差异。尽管欧盟检疫规定，在2000年至2004年进口到欧盟国家的47个针叶树样品中发现了生活PWNS。为了追踪引入葡萄牙的PWN种群的起源，随机扩增的多晶晶晶型DNA-PCR（RAPD-PCR ）进行以确定来自不同国家的PWN人群的遗传关系。

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