• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Forest Pathology >An improved method for qPCR detection of three Phytophthora spp. in forest and woodland soils in northern Britain
【24h】

An improved method for qPCR detection of three Phytophthora spp. in forest and woodland soils in northern Britain

机译：qPCR检测三种疫霉菌的改进方法。在英国北部的森林和林地土壤中

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Using TaqMan qPCR assays, DNA of P. ramorum, P. kernoviae and P. austrocedri was detected in 500 g soil samples collected from twelve infected forest and woodland sites in northern Britain. Phytophthora DNA was also amplified in soil adhering to boots after walking transects along footpaths or animal trails. At two sites, Phytophthora DNA was detected in soil over a 4-year period following removal of infected hosts. This new method enabling assessment of larger quantities of soil demonstrates the contamination risk of these pathogens in soil at infected sites and improves our understanding of the mechanisms of persistence and spread.
机译：使用TaqMan qPCR分析，在从英国北部十二个受感染的森林和林地收集到的500 g土壤样品中检测到了ramorum，P。kernoviae和Austrocedri的DNA。在沿着人行道或动物步道走样线后，附着在靴子上的土壤中疫霉菌DNA也被扩增。在两个地点，去除受感染宿主后的四年内，在土壤中检测到疫霉菌DNA。这种能够评估大量土壤的新方法证明了这些病原体在受感染地点土壤中的污染风险，并增进了我们对持久性和传播机制的理解。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号