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首页> 外文期刊>Forest Pathology >An effective medium for isolating Sphaeropsis sapinea from asymptomatic pines
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An effective medium for isolating Sphaeropsis sapinea from asymptomatic pines

机译：一种从无症状松树中分离出鞘脂的有效培养基

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The pathogen Sphaeropsis sapinea can persist in stems and branches of asymptomatic pines and can later induce disease when triggered by host stress. Several experiments were conducted to test if:(i) medium amended with tannic acid (TA) can increase the frequency of cultural detection of this shoot blight and canker pathogen from asymptomatic red pine (Pinus resinosa) stems, and (ii) S. sapinea can persist in asymptomatic red pine in the field following artificial inoculation. TA (0.5% w/v) in 2% (w/v) water agar proved to be the best medium for isolation of S. sapinea among a larger number of tested media. The addition of TA had little or no effect on the growth of two group A and two group B isolates of S. sapinea. However, when TA was added, 11 other fast-growing fungal isolates from stems/branches of red or jack pines (P. banksiana) were inhibited and grew more slowly (p < 0.05) than both S. sapinea groups. The TA-amended medium improved cultural detection of S. sapinea from 2-year-old, asymptomatic red pine nursery seedlings compared with two other methods used for the cultural detection of S. sapinea (32%vs. 8.5% and 18% recovery; p < 0.001 and p = 0.031, respectively). A field test using the TA-amended media established that S. sapinea can persist asymptomatically in red pine trees for at least 1 year. This medium significantly reduces the frequency of false-negatives from asymptomatic field material.
机译：病原体Spineeropsis sapinea可以存在于无症状松树的茎和枝中，并且在宿主压力触发后可以诱发疾病。进行了一些实验来测试是否：（i）用单宁酸（TA）改良的培养基可以提高无症状赤松（Pinus resinosa）茎上的该枯萎病和溃疡病病原体的培养检测频率，以及（ii）南美沙门氏菌人工接种后可在田间长期存在于无症状的红松中。在大量测试培养基中，在2％（w / v）水琼脂中的TA（0.5％w / v）被证明是分离茄形葡萄球菌的最佳培养基。 TA的添加对S. sapinea的两个A组和两个B组分离株的生长几乎没有影响。但是，当添加TA时，来自红色或杰克松（P. banksiana）的茎/枝的其他11种快速生长的真菌分离物被抑制，并且比两个南美番荔枝组生长更慢（p <0.05）。经TA改良的培养基与2种无症状赤松苗培养检测方法相比，可改善2岁无症状赤松苗圃苗中的南美白屈菜的培养检测（回收率分别为8.5％和18％; 32％）。 p <0.001，p = 0.031）。使用经TA改良的培养基进行的田间试验确定，南美番荔枝可以无症状地在赤松中持续至少1年。这种介质可显着降低无症状野外试验材料产生假阴性的频率。

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