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首页> 外文期刊>Mycologia >Persistence of Sphaeropsis sapinea on or in asymptomatic shoots of red and jack pines.
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Persistence of Sphaeropsis sapinea on or in asymptomatic shoots of red and jack pines.

机译：红色和千斤顶松的无症状芽上或中持续存在Sphaeropsis sapinea。

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摘要

To investigate the possibility that the conifer shoot blight and canker pathogen S. sapinea [Diplodia pinea] persists on or in asymptomatic red pine (Pinus resinosa) and jack pine (P. banksiana), dormant shoots were collected from nursery seedlingsand forest trees in Wisconsin, USA. One-hundred asymptomatic red pine seedlings were collected in November 1994 in each of 2 nurseries and 100 asymptomatic jack pine seedlings were collected at 1 of the nurseries. Fifty shoots of each species were collected at each of 3 forest sites. Each shoot was divided into 4 subsamples: 5 needle pairs from the current year's growth, 5 needle pairs from the previous year's growth, a stem segment from the current year's growth and a stem segment from the previous year's growth. Subsamples were surface-disinfested, placed into water agar slants and incubated for approximately 3 months. Identification of D. pinea was based on examination of spores from pycnidia that formed on the plant tissue. The fungus was associated with 27.5% of the red pine nursery seedlings but with none of the jack pine seedlings. With only 1 exception, the fungus was associated with stem segments of the previous year's growth. On samples collected in the forest, the pathogen was associated with both years' stem segments and with previous year's needles of each host. Characterization of isolates by means of RAPD marker analysis indicated presence of both A and B morphotypes of D. pinea which were confirmed as virulent by inoculation of red and jack pines, respectively. The ability of virulent strains to persist on or in asymptomatic hosts may help explain pathogen survival and rapid disease development under conditions that induce host stress.
机译：为了调查针叶树枯萎病和溃疡病病原体沙棘（Diplodia pinea）在无症状的红松（Pinus resinosa）和杰克松（P. banksiana）上持续存在的可能性，从威斯康星州的苗木和林木中收集了休眠的芽， 美国。 1994年11月在2个苗圃中分别收集了100棵无症状的红松苗，在其中1个苗圃中收集了100棵无症状的杰克松苗。在3个森林地点中的每个地点收集了50种每种植物的芽。每个芽被分为4个子样本：来自本年度增长的5针对，来自上一年增长的5针对，本年度增长的茎干和上一年增长的茎干。对子样品进行表面消毒，将其置于水琼脂斜面上并孵育约3个月。松果线虫的鉴定是基于检查在植物组织上形成的吡虫的孢子。该真菌与赤松苗圃苗的27.5％相关，但与杰克松苗无关。除1个例外，真菌与上年生长的茎部分有关。在森林中收集的样本中，病原体与两年的茎段和每个寄主的前一年的针叶相关。通过RAPD标记分析对分离物进行表征，表明存在松果线虫的A和B两种形态，分别通过接种红色和杰克松确认其为强毒。毒株在无症状宿主上或之中持续存在的能力可能有助于解释在诱发宿主压力的条件下病原体的存活和疾病的快速发展。

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