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首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >Endurance training alters antioxidant enzyme gene expression in rat skeletal muscle.
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Endurance training alters antioxidant enzyme gene expression in rat skeletal muscle.

机译：耐力训练会改变大鼠骨骼肌中抗氧化酶基因的表达。

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The effects of endurance training on gene expression of superoxide dismutase (SOD) and glutathione peroxidase (GPX) were investigated in type 2a and 2b skeletal muscles, as well as heart and liver, in the rat. Female Sprague-Dawley rats (4 months old, 300-320 g) were randomly divided into a trained (T, n = 11) and a control (C, n = 10) group and were pair fed a diet consisting of 66% cornstarch and 34% basal diet that contained all essential nutrients. Training was conducted on a treadmill at 25 m x min(-1), 10% grade for 2 h per day, 5 days per week for 10 weeks, resulting in a 79% (p < 0.01) increase in citrate synthase activity in the deep portion of vastus lateralis muscle (DVL, type 2a). Cu-Zn SOD activity was 35% higher (p < 0.01) in DVL of T versus C rats, and Cu-Zn SOD mRNA abundance showed a 125% increase with training (p < 0.05). Cu-Zn SOD protein content was not altered in DVL, but increased significantly (p < 0.05) in the superficial portion of vastus lateralis (type 2b) with training. Trained rats showed a 66% higher (p < 0.05) Mn SOD protein content in DVL, but Mn SOD activity and mRNA abundance were not affected. Training also significantly increased GPX activity by 62% (p < 0.05), without changing its mRNA abundance, in the DVL. Heart and liver showed a 112 and 58% increase (p < 0.01) in Cu-Zn SOD mRNA abundance with training, respectively, but no other training adaptation was detected. These data indicate that endurance training can promote gene expression of muscle antioxidant enzymes in a fiber-specific manner. Training appears to upregulate Cu-Zn SOD mRNA abundance in a number of aerobic tissues, whereas Mn SOD and GPX induction observed in DVL may occur at the post-transcriptional levels.
机译：在大鼠2a和2b型骨骼肌以及心脏和肝脏中，研究了耐力训练对超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPX）基因表达的影响。将雌性Sprague-Dawley大鼠（4个月大，300-320 g）随机分为训练组（T，n = 11）和对照组（C，n = 10），并配对饲喂66％的玉米淀粉含所有必需营养素的基础饮食占34％。在跑步机上以25 mx min（-1），10％的坡度每天进行2小时，每周5天，持续10周的训练，结果是深层柠檬酸合酶活性提高了79％（p <0.01）股外侧肌的一部分（DVL，2a型）。 T大鼠的DVL中的Cu-Zn SOD活性比C大鼠高35％（p <0.01），而Cu-Zn SOD mRNA的丰度在训练后增加了125％（p <0.05）。在训练中，铜-锌超氧化物歧化酶的蛋白质含量在DVL中没有改变，但在外侧股外侧浅表部分（2b型）中显着增加（p <0.05）。受过训练的大鼠显示DVL中的Mn SOD蛋白含量高66％（p <0.05），但Mn SOD活性和mRNA丰度不受影响。在DVL中，训练还可以显着提高GPX活性62％（p <0.05），而不会改变其mRNA丰度。通过训练，心脏和肝脏的Cu-Zn SOD mRNA丰度分别增加了112％和58％（p <0.01），但未检测到其他训练适应性。这些数据表明，耐力训练可以以纤维特异性的方式促进肌肉抗氧化酶的基因表达。训练似乎可以上调许多需氧组织中Cu-Zn SOD mRNA的含量，而在DVL中观察到的Mn SOD和GPX诱导可能在转录后水平发生。

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