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首页> 外文期刊>Foodborne pathogens and disease >Presence of shiga toxin-producing escherichia coli o-groups in small and very-small beef-processing plants and resulting ground beef detected by a multiplex polymerase chain reaction assay
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Presence of shiga toxin-producing escherichia coli o-groups in small and very-small beef-processing plants and resulting ground beef detected by a multiplex polymerase chain reaction assay

机译：在小型和小型牛肉加工厂中存在产生志贺毒素的大肠杆菌o-基团，并通过多重聚合酶链反应测定法检测到所得碎牛肉

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摘要

Shiga toxin-producing Escherichia coli (STEC) are associated with foodborne illnesses, including hemolytic uremic syndrome in humans. Cattle and consequently, beef products are considered a major source of STEC. E. coli O157:H7 has been regulated as an adulterant in ground beef since 1996. The United States Department of Agriculture Food Safety and Inspection Service began regulating six additional STEC (O145, O121, O111, O103, O45, and O26) as adulterants in beef trim and raw ground beef in June 2012. Little is known about the presence of STEC in small and very-small beef-processing plants. Therefore, we propose to determine whether small and very-small beef-processing plants are a potential source of non-O157:H7 STEC. Environmental swabs, carcass swabs, hide swabs, and ground beef from eight small and very-small beef-processing plants were obtained from October 2010 to December 2011. A multiplex polymerase chain reaction assay was used to determine the presence of STEC O-groups: O157, O145, O121, O113, O111, O103, O45, and O26 in the samples. Results demonstrated that 56.6% (154/272) of the environmental samples, 35.0% (71/203) of the carcass samples, 85.2% (23/27) of the hide samples, and 17.0% (20/118) of the ground beef samples tested positive for one or more of the serogroups. However, only 7.4% (20/272) of the environmental samples, 4.4% (9/203) of the carcass samples, and 0% (0/118) ground beef samples tested positive for both the serogroup and Shiga toxin genes. Based on this survey, small and very-small beef processors may be a source of non-O157:H7 STEC. The information from this study may be of interest to regulatory officials, researchers, public health personnel, and the beef industry that are interested in the presence of these pathogens in the beef supply.
机译：产生志贺毒素的大肠杆菌（STEC）与食源性疾病有关，包括人类的溶血性尿毒症综合征。牛，因此，牛肉产品被认为是STEC的主要来源。自1996年以来，已将大肠杆菌O157：H7掺入碎牛肉中进行掺假。美国农业部食品安全和检验局开始对另外六个STEC（O145，O121，O111，O103，O45和O26）进行掺假。于2012年6月在牛肉条和生碎牛肉中使用。在小型和小型牛肉加工厂中STEC的存在鲜为人知。因此，我们建议确定小型和超小型牛肉加工厂是否是非O157：H7 STEC的潜在来源。从2010年10月至2011年12月，从8家小型和超小型牛肉加工厂获得了环境棉签，cas体棉签，生皮棉签和碎牛肉。采用多重聚合酶链反应测定法确定STEC O-基团的存在：样品中的O157，O145，O121，O113，O111，O103，O45和O26。结果表明，环境样品中的56.6％（154/272），car体样品中的35.0％（71/203），生皮样品中的85.2％（23/27）和地面的17.0％（20/118）牛肉样品检测到一个或多个血清群呈阳性。但是，只有7.4％（20/272）的环境样品，4.4％（9/203）的car体样品和0％（0/118）的碎牛肉样品对血清群和志贺毒素基因均呈阳性。根据此调查，小型和小型牛肉加工者可能是非O157：H7 STEC的来源。监管官员，研究人员，公共卫生人员和牛肉行业可能会对本研究中的信息感兴趣，这些官员对牛肉供应中这些病原体的存在感兴趣。

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