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首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >Modulation of radiosensitivity in Chinese hamster lung fibroblasts by cisplatin.
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Modulation of radiosensitivity in Chinese hamster lung fibroblasts by cisplatin.

机译：顺铂对中国仓鼠肺成纤维细胞放射敏感性的调节。

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摘要

The effects of cisplatin exposure time, concentration, and irradiation sequence on the sensitivity of Chinese hamster lung fibroblasts (V79) to gamma-ray exposure were examined. Based on clonogenic cell survival, the cisplatin concentrations corresponding to 50% cell survival (EC(50)) for exposure times of 1 h to 7 days followed a 2-phase exponential decay and ranged from 28.26 +/- 3.32 to 1.53 +/- 0.24 micromol/L, respectively. When cells were treated at EC(50) for exposures of less than 4 h and irradiated immediately, cisplatin inhibited the effect of radiation. Exposures of 4-6 h did not affect radiosensitivity. For exposures of 8-12 h, radiosensitization was observed, which disappeared at 14 h and reappeared for much longer cisplatin treatments. At the lowest achievable EC(50) (1.53 micromol/L), radiosensitization was observed if irradiation was delayed for 1-8 h. This enhancement in radiosensitivity disappeared for irradiation delays of 10-12 h, but reappeared when irradiation was delayed for 14-18 h. These data demonstrate that the mode of interaction between cisplatin and gamma-irradiation depends on the concentration and exposure time of cisplatin, as well as on the timing of irradiation after cisplatin administration. Consideration of changes in cell cycle kinetics may contribute to the improvement of treatment outcomes in adjuvant chemoradiotherapy involving cisplatin.
机译：考察了顺铂暴露时间，浓度和照射顺序对中国仓鼠肺成纤维细胞（V79）对γ射线敏感性的影响。根据成克隆细胞存活率，在1小时至7天的暴露时间内，对应于50％细胞存活率（EC（50））的顺铂浓度随两相指数衰减而变化，范围为28.26 +/- 3.32至1.53 +/-分别为0.24 micromol / L。当细胞在EC（50）下处理少于4小时并立即照射时，顺铂抑制了放射作用。暴露4-6小时不影响放射敏感性。对于8-12小时的照射，观察到放射增敏作用，在14小时时消失，并在更长的顺铂治疗中再次出现。在可达到的最低EC（50）（1.53 micromol / L）下，如果将辐射延迟1-8小时，则会观察到放射增敏作用。放射敏感性的这种增强对于辐射延迟10-12小时消失了，但是当辐射延迟14-18小时后又出现了。这些数据表明，顺铂与伽马射线辐射之间的相互作用方式取决于顺铂的浓度和暴露时间，以及顺铂给药后的照射时间。考虑细胞周期动力学变化可能有助于改善涉及顺铂的辅助放化疗中的治疗结果。

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