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首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >Suppression of LPS-induced inflammatory responses by inflexanin B in BV2 microglial cells
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Suppression of LPS-induced inflammatory responses by inflexanin B in BV2 microglial cells

机译：inflexanin B在BV2小胶质细胞中抑制LPS诱导的炎症反应

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摘要

Microglia are a type of resident macrophage that functions as an inflammation modulator in the central nervous system. Over-activation of microglia by a range of stimuli disrupts the physiological homeostasis of the brain, and induces inflammatory response and degenerative processes, such as those implicated in neurodegenerative diseases, including Alzheimer's disease and Parkinson's disease. Therefore, we investigated the possible anti-inflammatory mechanisms of inflexanin B in murine microglial BV2 cells. Lipopolysaccharide (LPS) activated BV2 cells and induced the production of pro-inflammatory mediators such as nitric oxide (NO), prostaglandin E2 (PGE2), and cytokines (interleukins-1β and -6, and tumour necrosis factor α). The LPS-induced production of pro-inflammatory mediators was associated with the enhancement of nuclear factor-kappaB (NF-κB) nuclear translocation and the activation of mitogen-activated protein kinase (MAPK) including ERK1/2 and JNK. Conversely, pretreatment of cells with inflexanin B (10 and 20 μg/mL) significantly reduced the production of pro-inflammatory mediators. This was accompanied with the reduced nuclear translocation of NF-κB and reduced activation of MAPKs. These results suggest that inflexanin B attenuated the LPS-induced inflammatory process by inhibiting the activation of NF-κB and MAPKs.
机译：小胶质细胞是常驻巨噬细胞，在中枢神经系统中起炎症调节剂的作用。小胶质细胞被一系列刺激过度激活会破坏大脑的生理稳态，并引起炎症反应和变性过程，例如与神经退行性疾病（包括阿尔茨海默氏病和帕金森氏病）有关的过程。因此，我们调查了inflexanin B在小鼠小胶质细胞BV2细胞中可能的抗炎机制。脂多糖（LPS）激活BV2细胞并诱导产生促炎性介质，例如一氧化氮（NO），前列腺素E2（PGE2）和细胞因子（白介素-1β和-6，以及肿瘤坏死因子α）。 LPS诱导的促炎性介质的产生与核因子-κB（NF-κB）核易位的增强以及丝裂原活化蛋白激酶（MAPK）的激活有关，包括ERK1 / 2和JNK。相反，用inflexanin B（10和20μg/ mL）预处理细胞会显着降低促炎性介质的产生。这伴随着NF-κB核转运减少和MAPKs活化减少。这些结果表明，inflexanin B通过抑制NF-κB和MAPKs的激活来减弱LPS诱导的炎症过程。

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