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首页> 外文期刊>Gene expression >Inhibition of translation by consecutive rare leucine codons in E. coli: absence of effect of varying mRNA stability.
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Inhibition of translation by consecutive rare leucine codons in E. coli: absence of effect of varying mRNA stability.

机译：连续的稀有亮氨酸密码子在大肠杆菌中的翻译抑制：不存在变化的mRNA稳定性的影响。

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摘要

Consecutive homologous codons that are rarely used in E. coli are known to inhibit translation to varying degrees. As few as two consecutive rare arginine codons exhibit a profound inhibition of translation when they are located in the 5' portion of a gene in E. coli. We have previously shown that nine consecutive rare CUA leucine codons cause almost complete inhibition of translation when they are placed after the 13th codon of a test message (although they do not inhibit translation when they are placed in the middle of the message). In the present work, we report that five consecutive rare CUA leucine codons exhibit approximately a threefold inhibition of translation when they are similarly placed after the 13th codon of a test message, compared to five consecutive common CUG leucine codons, in a T7 RNA polymerase-driven system. Further, by removing RNase III processing sites at the 3' ends of the mRNAs, we have manipulated the stability of the mRNAs encoding the test and control messages to see if decreasing mRNA stability might have an effect on the extent of translation inhibition by the rare leucine codons. However, the inhibition with the less stable mRNAs was similar to that with the stable mRNAs, approximately 3.4-fold, indicating that mRNA stability per se does not have a major influence on the effects of rare codons in this system.
机译：众所周知，在大肠杆菌中很少使用的连续同源密码子会不同程度地抑制翻译。当位于大肠杆菌中基因的5'部分时，少至两个连续的稀有精氨酸密码子就表现出对翻译的深刻抑制。先前我们已经表明，当将9个连续的稀有CUA亮氨酸密码子置于测试消息的第13个密码子之后时，它们几乎完全抑制翻译（尽管当它们置于消息中间时，它们不抑制翻译）。在目前的工作中，我们报告了在T7 RNA聚合酶中，与五个连续的常见CUG亮氨酸密码子相比，五个连续的稀有CUA亮氨酸密码子在测试消息的第13个密码子之后相似地放置时，表现出大约三倍的翻译抑制。驱动系统。此外，通过去除mRNA的3'端的RNase III加工位点，我们操纵了编码测试和控制信息的mRNA的稳定性，以查看降低的mRNA稳定性是否可能会对这种罕见的翻译抑制程度产生影响。亮氨酸密码子。然而，不稳定的mRNA的抑制作用与稳定的mRNA的抑制作用相似，约为3.4倍，这表明mRNA的稳定性本身对该系统中稀有密码子的作用没有重大影响。

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