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首页> 外文期刊>Fish & Shellfish Immunology >Molecular characterization of hepcidin gene in common carp (Cyprinus carpio L.) and its expression pattern responding to bacterial challenge
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Molecular characterization of hepcidin gene in common carp (Cyprinus carpio L.) and its expression pattern responding to bacterial challenge

机译：鲤鱼中铁调素基因的分子特征及其对细菌攻击的表达模式

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摘要

Hepcidin is a cysteine-rich cationic antimicrobial peptide (AMP), which plays an important role in host innate immune system and iron regulation. A great many of hepcidin genes have been identified from vertebrates, including various fish species. Here we report the cloning and identification of a hepcidin cDNA from the liver of common carp (Cyprinus carpi L.). The full-length cDNA of the common carp hepcidin was 647 bp, which contained an ORF of 276 bp encoding a prepropeptide of 91 amino acid residues. The predicted preprohepcidin consisted of three domains: a signal peptide of 24 amino acids, a prodomain of 42 amino acids and a mature peptide of 25 amino acids, which containd eight cysteine residues at the identical conserved position. The genomic organization of common carp hepcidin was identified, which contained three exons and two introns, similarly to corresponding genes in mammals and other fish species. Sequence alignment and phylogenetic analysis showed that hepcidins were conserved in different species, and common carp hepcidin was type 1 hepcidin according to the phylogenetic tree, which had the highest identity with mud loach and zebrafish. Real-time PCR assay showed that the hepcidin gene was mostly expressed in liver, and expressed in all the other tested tissues of common carp in different levels. When challenged with pathogenic bacterium, Vibrio anguillarum, the expression level of common carp hepcidin was quickly up-regulated in liver, spleen, head kidney and hindgut, implying that hepcidin may be an important component of the innate immune system of common carp and involved in mucosal immune response against invading pathogens. (C) 2013 Elsevier Ltd. All rights reserved.
机译：Hepcidin是富含半胱氨酸的阳离子抗菌肽（AMP），在宿主先天免疫系统和铁调节中起重要作用。已经从脊椎动物中鉴定出许多铁调素基因，包括各种鱼类。在这里，我们报道了从鲤鱼肝脏中的铁调素cDNA的克隆和鉴定。鲤鱼hepcidin的全长cDNA为647 bp，其ORF为276 bp，编码91个氨基酸残基的前肽。预测的前肝素原由三个结构域组成：24个氨基酸的信号肽，42个氨基酸的前结构域和25个氨基酸的成熟肽，它们在相同的保守位置包含8个半胱氨酸残基。确定了鲤鱼hepcidin的基因组组织，其中包含三个外显子和两个内含子，与哺乳动物和其他鱼类的相应基因相似。序列比对和系统进化分析表明，铁皮素在不同物种中是保守的，根据系统发育树，鲤鱼铁皮素为1型铁皮素，与泥lo和斑马鱼具有最高的同一性。实时荧光定量PCR检测表明，hepcidin基因主要在肝脏中表达，并在不同水平的鲤鱼的所有其他受试组织中表达。当用致病菌鳗弧菌攻击时，鲤鱼肝素在肝脏，脾脏，头部肾脏和后肠中的表达水平迅速上调，这暗示着肝素可能是鲤鱼先天免疫系统的重要组成部分，并参与其中。黏膜对入侵病原体的免疫反应。 （C）2013 Elsevier Ltd.保留所有权利。

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