Molecular and functional characterizations of a Kunitz-type serine protease inhibitor FcKuSPI of the shrimp Fenneropenaeus chinensis

Kong Hee Jeong; Lee Ye-Ji; Park In-Suk; Lee Won Woo; Kim Young-K; Nam Bo-Hye; Kim Woo-Jin; Jung Hyungtaek; Jeon You-Jin; An Cheul Min; Lee Sang-Jun

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Molecular and functional characterizations of a Kunitz-type serine protease inhibitor FcKuSPI of the shrimp Fenneropenaeus chinensis

机译：虾粉对虾的Kunitz型丝氨酸蛋白酶抑制剂FcKuSPI的分子和功能表征

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Serine proteinase inhibitors play important and diverse roles in biological processes such as coagulation, defense mechanisms, and immune responses. Here, we identified and characterized a Kunitz-type proteinase inhibitor, designated FcKuSPI, of the BPTI/Kunitz family of serine proteinase inhibitors from the hemocyte cDNA library of the shrimp Fenneropenaeus chinensis. The deduced amino acid sequence of FcKuSPI comprises 80 residues with a putative signal peptide of 15 amino acids. The predicted molecular weight of the mature peptide is 7.66 kDa and its predicted isoelectric point is 8.84. FcKuSPI includes a Kunitz domain containing six conserved cysteine residues that are predicted to form three disulfide bonds. FcKuSPI shares 44-53% homology with BPTI/Kunitz family members from other species. FcKuSPI mRNA was expressed highly in the hemocytes and moderately in muscle in healthy shrimp. Recombinant FcKuSPI protein demonstrated anti-protease activity against trypsin and anticoagulant activity against citrated human plasma in a dose-dependent manner in in vitro assays. (C) 2013 Elsevier Ltd. All rights reserved.

机译：丝氨酸蛋白酶抑制剂在凝血，防御机制和免疫反应等生物学过程中发挥着重要而多样的作用。在这里，我们从虾粉红色对虾的血细胞cDNA文库中鉴定并鉴定了BPTI / Kunitz丝氨酸蛋白酶抑制剂家族的Kunitz型蛋白酶抑制剂，称为FcKuSPI。推导的FcKuSPI氨基酸序列包含80个残基，推定的信号肽包含15个氨基酸。成熟肽的预测分子量为7.66 kDa，其预测等电点为8.84。 FcKuSPI包含一个Kunitz域，其中包含六个保守的半胱氨酸残基，预计将形成三个二硫键。 FcKuSPI与其他物种的BPTI / Kunitz家族成员具有44-53％的同源性。在健康虾中，FcKuSPI mRNA在血细胞中高表达，在肌肉中适度表达。重组FcKuSPI蛋白在体外测定中呈剂量依赖性，显示出对胰蛋白酶的抗蛋白酶活性和对柠檬酸人血浆的抗凝活性。（C）2013 Elsevier Ltd.保留所有权利。

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《Fish & Shellfish Immunology》 |2013年第3期|共5页
作者
Kong Hee Jeong; Lee Ye-Ji; Park In-Suk; Lee Won Woo; Kim Young-K; Nam Bo-Hye; Kim Woo-Jin; Jung Hyungtaek; Jeon You-Jin; An Cheul Min; Lee Sang-Jun;
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正文语种 eng
中图分类水产、渔业;
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Kunitz-type; Serine proteinase inhibitor; Fenneropenaeus chinensis; Hemocyte; Anticoagulant;

机译：Kunitz型丝氨酸蛋白酶抑制剂猪鞭草血红细胞抗凝;

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1. Molecular and functional characterizations of a Kunitz-type serine protease inhibitor FcKuSPI of the shrimp Fenneropenaeus chinensis [J] . Kong Hee Jeong, Lee Ye-Ji, Park In-Suk, Fish & Shellfish Immunology . 2013,第3期

机译：虾粉对虾的Kunitz型丝氨酸蛋白酶抑制剂FcKuSPI的分子和功能表征
2. Structures of a bi-functional Kunitz-type STI family inhibitor of serine and aspartic proteases: Could the aspartic protease inhibition have evolved from a canonical serine protease-binding loop? [J] . Guerra Yasel, Valiente Pedro A., Pons Tirso, Journal of Structural Biology . 2016,第2期

机译：丝氨酸和天冬氨酸蛋白酶的双功能Kunitz型STI家族抑制剂的结构：天冬氨酸蛋白酶抑制作用是否可以从典型的丝氨酸蛋白酶结合环发展而来？
3. Expression of four trypsin-like serine proteases from the Chinese shrimp, Fenneropenaeus chinensis, as regulated by pathogenic infection [J] . Shi XZ, Ren Q, Zhao XF, Comparative biochemistry and physiology, Part B. Biochemistry & molecular biology . 2009,第1期

机译：中国虾对虾中四种胰蛋白酶样丝氨酸蛋白酶的表达受致病菌感染的调控
4. Structure-based .Functional Characterization of Oryctin, a Hemolymph Protein from the Coconut Rhinoceros Beetle, Oryctes Rhinoceros, As a Novel Serine Protease Inhibitor [C] . Shoichiro Horita, Jun Ishibashi, Koji Nagata Japanese peptide symposium . 2011

机译：基于结构的。Oryctin的函数表征，来自椰子鼻窦甲虫的血淋巴蛋白，Oryctes rhinoceros，作为一种新的丝氨酸蛋白酶抑制剂
5. Linked equilibria in protein-protein binding: Thermodynamic modeling of contributions to the binding of a serine protease inhibitor to a serine protease. [D] . Horn, James Richard. 2002

机译：蛋白质-蛋白质结合中的链接平衡：对丝氨酸蛋白酶抑制剂与丝氨酸蛋白酶结合的贡献的热力学模型。
6. Molecular Characterization of Ancylostoma ceylanicum Kunitz-Type Serine Protease Inhibitor: Evidence for a Role in Hookworm-Associated Growth Delay [O] . Daniel Chu, Richard D. Bungiro, Maureen Ibanez, 2004

机译：棘皮动物Anylylostoma ceylanicum Kunitz型丝氨酸蛋白酶抑制剂的分子表征：在钩虫相关的生长延迟中作用的证据。
7. Structures of a bi-functional Kunitz-type STI family inhibitor of serine and aspartic proteases: could the aspartic protease inhibition have evolved from a canonical serine protease-binding loop? [O] . Guerra Yasel, Valiente Pedro A., Pons Tirso, 2016

机译：丝氨酸和天冬氨酸蛋白酶的双功能Kunitz型STI家族抑制剂的结构：天冬氨酸蛋白酶抑制作用是否可以从典型的丝氨酸蛋白酶结合环发展而来？
8. Epithelial-Derived, Integral Membrane, Kunitz-Type Serine Protease Inhibitor in Breast Cancer [R] . Lin, C. 2005

机译：上皮衍生的整合膜，Kunitz型丝氨酸蛋白酶抑制剂在乳腺癌中的应用

Molecular and functional characterizations of a Kunitz-type serine protease inhibitor FcKuSPI of the shrimp Fenneropenaeus chinensis

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