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首页> 外文期刊>Fish & Shellfish Immunology >Dietary choline modulates immune responses, and gene expressions of TOR and eIF4E-binding protein2 in immune organs of juvenile Jian carp (Cyprinus carpio var. Jian)
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Dietary choline modulates immune responses, and gene expressions of TOR and eIF4E-binding protein2 in immune organs of juvenile Jian carp (Cyprinus carpio var. Jian)

机译：日粮胆碱调节幼年Jian鱼免疫器官的免疫反应以及TOR和eIF4E结合蛋白2的基因表达

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摘要

The present work evaluates the effects of various levels of dietary choline on immune parameters, immune-related gene expression and protection against Aeromonas hydrophila (AH) in juvenile Jian carp (Cyprinus carpio var. Jian). Fish were fed with six different experimental diets containing graded levels of choline at 165 (choline-deficient control), 310, 607, 896, 1167 and 1820 mg kg(-1) diet for 65 days. At the end of the feeding trail, Fish were challenged with AH and mortalities were recorded over 17 days. Dietary choline significantly enhanced spleen and head kidney weights, spleen index, red blood cell and white blood cell counts, and intestinal Lactobacillus counts of juvenile Jian carp; whereas, intestinal Escherichia coli and A. hydrophila counts decreased. Moreover, the post-challenge survival rate, leucocyte phagocytic capacity, serum lysozyme and acid phosphatase activities, hemagglutination titer, complement 3 and 4 contents, immunoglobulin M content, and anti-AM antibody titer were significantly enhanced by choline and the lowest in choline-deficient group, while serum total iron-binding capacity was the highest in choline-deficient group. The relative gene expressions of interleukin 10 in spleen and head kidney, target of rapamycin (TOR) in spleen and eIF4E-binding protein2 (4E-BP2) in head kidney significantly increased with increasing of dietary choline up to a certain point. However, the relative gene expressions of interleukin 1 beta, tumor necrosis factor alpha and transforming growth factor beta 2 in spleen and head kidney, TOR in head kidney and 4E-BP2 in spleen significantly decreased. In conclusion, dietary choline improved disease resistance, enhanced the immune function, and regulated immune-related gene expression of juvenile Jian carp. (C) 2013 Elsevier Ltd. All rights reserved.
机译：本工作评估了不同水平的饮食胆碱对幼年建鲤（Cyprinus carpio var。Jian）的免疫参数，免疫相关基因表达和对嗜水气单胞菌（AH）的保护作用。用六种不同的实验饮食喂鱼，这些饮食在165天（胆碱缺乏对照），310、607、896、1167和1820 mg kg（-1）的饮食中具有分级的胆碱水平，持续65天。喂食步道结束时，鱼受到AH攻击，并在17天内记录了死亡人数。饮食胆碱可显着提高鲤鱼的脾脏和头部肾脏重量，脾脏指数，红细胞和白细胞计数以及肠道乳杆菌计数;而肠道大肠杆菌和亲水曲霉菌计数下降。此外，胆碱显着提高了攻击后的存活率，白细胞吞噬能力，​​血清溶菌酶和酸性磷酸酶活性，血凝滴度，补体3和4含量，免疫球蛋白M含量以及抗AM抗体滴度，而胆碱最低。缺乏组，而血清总铁结合能力在胆碱缺乏组最高。随着饮食中胆碱含量的增加，脾脏和头部肾脏中白介素10的相对基因表达，脾脏中雷帕霉素靶标（TOR）和头部肾脏中eIF4E结合蛋白2（4E-BP2）的相对表达显着增加。然而，脾脏和头肾中白细胞介素1β，肿瘤坏死因子α和转化生长因子β2的相对基因表达，头肾中的TOR和脾脏中的4E-BP2的相对基因表达显着降低。总之，日粮胆碱可改善Jian鱼的抗病性，增强其免疫功能，并调节其免疫相关基因的表达。 （C）2013 Elsevier Ltd.保留所有权利。

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