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首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >Cytosolic fatty acid binding protein enhances rat hepatocyte (3H)palmitate uptake.
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Cytosolic fatty acid binding protein enhances rat hepatocyte (3H)palmitate uptake.

机译：胞质脂肪酸结合蛋白可增强大鼠肝细胞（3H）棕榈酸酯的摄取。

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摘要

Liver cytosolic fatty acid binding protein (FABP) represents the intracellular equivalent to extracellular serum albumin, participating in the intracellular transport of long-chain fatty acids. In this study we observed the effect of increasing and decreasing FABP levels on hepatocyte [3H]palmitate uptake in male Sprague-Dawley rats. We also were interested to determine whether uptake, from either the unbound or unbound and protein-bound fractions, was fundamentally different at the different FABP levels. FABP levels were modified by hypophysectomy and clofibrate treatment (50 mg/100 g body weight for 10 days). Results showed that the [3H]palmitate clearance rates paralleled the 54% decrease and 73% increase in FABP levels in hypophysectomy and clofibrate-treated animals, respectively. In the presence of 2 and 20 microM albumin, hepatocyte clearance rates of unbound [3H]palmitate from hypophysectomized animals (0.16+/-0.01 and 0.64+/-0.01 mL x s(-1) x 10(-6) cells, respectively) were significantly lower (p<0.01) than those of the sham group (0.30+/-0.02 and 1.00+/-0.06 mL x s(-1) x 10(-6) cells, respectively). However, the unbound [3H]palmitate clearance rates from the clofibrate-treated group (0.39+/-0.04 and 1.18+/-0.12 mL x s(-1) x 10(-6) cells) were significantly higher (p<0.01) than the control group (0.29+/-0.02 and 0.81+/-0.05 mL x s(-1) x 10(-6) cells) for 2 and 20 microM albumin, respectively. To investigate whether uptake was fundamentally different between the hypophysectomized and clofibrate-treated groups, we expressed the clearance rates as enhancement factors, i.e., EF = CL20 microM/CL2microM. No statistical difference was observed between EF of the hypophsectomized (3.8+/-0.4) and EF of the clofibrate-treated (3.1+/-0.3) groups, suggesting that the extracted ligand originated from similar fractions.
机译：肝细胞内脂肪酸结合蛋白（FABP）代表细胞内等同于细胞外血清白蛋白，参与长链脂肪酸的细胞内转运。在这项研究中，我们观察到了增加和降低FABP水平对雄性Sprague-Dawley大鼠肝细胞[3H]棕榈酸酯摄取的影响。我们也有兴趣确定在不同的FABP水平下，未结合或未结合及蛋白质结合部分的摄取是否根本不同。通过垂体切除和氯贝特治疗（50 mg / 100 g体重，持续10天）可以改变FABP的水平。结果表明，[3H]棕榈酸酯清除率分别与垂体切除术和经氯贝特治疗的动物的FABP水平分别降低了54％和73％。在存在2和20 microM白蛋白的情况下，来自切除后切除的动物的未结合[3H]棕榈酸酯的肝细胞清除率（分别为0.16 +/- 0.01和0.64 +/- 0.01 mL xs（-1）x 10（-6）细胞）显着低于假手术组（p <0.01）（分别为0.30 +/- 0.02和1.00 +/- 0.06 mL xs（-1）x 10（-6）细胞）。然而，氯贝贝特治疗组的未结合的[3H]棕榈酸酯清除率（0.39 +/- 0.04和1.18 +/- 0.12 mL xs（-1）x 10（-6）细胞）明显更高（p <0.01）与对照组相比（0.29 +/- 0.02和0.81 +/- 0.05 mL xs（-1）x 10（-6）细胞）分别获得2和20 microM白蛋白。为了研究经hyperphytomtomized和氯贝贝特治疗组之间的摄取是否有根本不同，我们将清除率表示为增强因子，即EF = CL20 microM / CL2microM。经低位切除的（3.8 +/- 0.4）的EF和经氯贝特治疗的（3.1 +/- 0.3）组的EF之间没有观察到统计学差异，表明提取的配体源自相似的馏分。

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