...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >Na+ channel and Na+-K+ ATPase involvement in norepinephrine- and veratridine-stimulated metabolism in perfused rat hind limb.
【24h】

Na+ channel and Na+-K+ ATPase involvement in norepinephrine- and veratridine-stimulated metabolism in perfused rat hind limb.

机译：Na +通道和Na + -K + ATPase参与去甲肾上腺素和藜芦定刺激的大鼠后肢新陈代谢。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In the constant flow perfused rat hind limb, norepinephrine (NE) evoked increases in oxygen uptake (VO2) and lactate efflux (LE) were inhibited by the cardiac glycoside ouabain (1 mM), without interrupting the NE-mediated vasoconstriction. The membrane labilizer veratridine, previously shown to increase VO2 and LE, without increasing perfusion pressure, was also shown to be inhibited by the cardiac glycoside ouabain, as well as by the ouabain analogues digitoxin and digoxin. The stimulatory actions of veratridine on VO2 were inhibitable by low doses of the specific sodium channel blocker tetrodotoxin (TTX), while NE effects were unaffected, suggesting that NE may be acting via a TTX-insensitive sodium channel. It is concluded that agents such as NE (a vasoconstrictor) or veratridine (a membrane labilizer), which stimulate VO2 in the perfused rat hind limb, do so by increasing Na+ influx. The observed increases in oxygen consumption and LE are due to Na+-K+ ATPase activity to pump Na+ out of the cell at the expense of ATP turnover. Energy dissipation due to Na+ cycling may be a form of facultative thermogenesis attributable to NE that can be stimulated by membrane labilizers such as veratridine in the constant flow perfused rat hind limb.
机译：在恒流灌注的大鼠后肢中，强心苷哇巴因（1 mM）抑制了去甲肾上腺素（NE）引起的摄氧量（VO2）和乳酸外排（LE）的增加，而没有中断NE介导的血管收缩。先前证明在不增加灌注压力的情况下，可增加VO2和LE的膜Labilator Veratridine也可被强心苷哇巴因以及哇巴因类似物洋地黄毒苷和地高辛抑制。小剂量的特定钠通道阻滞剂河豚毒素（TTX）抑制了Vertridine对VO2的刺激作用，而NE的影响不受影响，这表明NE可能是通过TTX不敏感的钠通道起作用。可以得出结论，诸如NE（血管收缩剂）或verrridine（膜实验室增效剂）之类的药物可通过增加Na +流入量来刺激灌注的大鼠后肢中的VO2。观察到的耗氧量和LE的增加归因于Na + -K + ATPase的活性，使Na +泵出细胞而以ATP周转为代价。由于Na +循环而造成的能量耗散可能是NE引起的兼性生热的一种形式，可以通过膜流动器（如veratridine）在恒流灌注大鼠后肢中刺激NE。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号