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首页> 外文期刊>Fish & Shellfish Immunology >Protection of Macrobrachium rosenbergii against white tail disease by oral administration of bacterial expressed and encapsulated double-stranded RNA
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Protection of Macrobrachium rosenbergii against white tail disease by oral administration of bacterial expressed and encapsulated double-stranded RNA

机译：通过口服施用细菌表达和包裹的双链RNA保护罗氏沼虾免受白尾病

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摘要

White tail disease (WTD) of cultured Macrobrachium rosenbergii is caused by M. rosenbergii nodavirus (MrNV) and an extra small virus (XSV), both present together, and the mortality rate can be as high as 100% within 2 or 3 days of infection. Possible protection of M. rosenbergii against WTD by oral administration of bacterial expressed and encapsulated double-stranded RNA (dsRNA) was studied. juvenile M. rosenbergii were fed with the feed coated with inactivated bacteria encapsulated dsRNA of MrNV and XSV genes individually and in combination for 7 days followed by challenge with WTD causing agents at 24 h and 72 h post-feeding. Test animals fed with a combination of dsRNA of MrNV and XSV capsid genes showed the highest relative percent survival (RPS) when compared to other treatments with RPS of 80% and 75% at 24 and 72 h respectively. One hundred percent mortality was observed in test animals fed with control dsRNA coated feed. Although in the literature, injection is the most common method used to deliver dsRNA, this study shows that oral administration is effective, feasible and economical. (C) 2013 Elsevier Ltd. All rights reserved.
机译：培养的罗氏沼虾的白尾病（WTD）是由罗氏沼虾诺达病毒（MrNV）和超小型病毒（XSV）共同引起的，死亡率在感染后2或3天内高达100％感染。通过口服细菌表达和封装的双链RNA（dsRNA），研究了罗氏沼虾对WTD的可能保护作用。将罗氏沼虾幼体分别包被MrNV和XSV基因的灭活细菌包封的dsRNA包被的饲料7天，然后在喂食后24 h和72 h用WTD引发剂攻击。与分别在24小时和72小时的RPS分别为80％和75％的其他治疗方法相比，饲喂MrNV和XSV衣壳基因的dsRNA组合的测试动物表现出最高的相对存活百分比（RPS）。在用对照dsRNA包被的饲料喂养的试验动物中观察到百分之一百的死亡率。尽管在文献中，注射是用于递送dsRNA的最常用方法，但这项研究表明口服给药是有效，可行和经济的。 （C）2013 Elsevier Ltd.保留所有权利。

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