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首页> 外文期刊>Gene expression >Expression of genes involved in nitrogen assimilation and the C/N balance sensing in Prochlorococcus sp. strain SS120.
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Expression of genes involved in nitrogen assimilation and the C/N balance sensing in Prochlorococcus sp. strain SS120.

机译：在Prochlorococcus sp。中参与氮同化和C / N平衡感测的基因的表达。菌株SS120。

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摘要

The expression of five genes involved in nitrogen assimilation in cyanobacteria, namely glnA, glsF, icd, ntcA, and glnB, encoding three key enzymes from that pathway (glutamine synthetase, glutamate synthase, isocitrate dehydrogenase) and two regulatory proteins (NtcA and PII), was studied in this work. Their changes under different conditions were analyzed by quantitative real-time RT-PCR. Nutrient limitation induced clear modifications on the expression of most studied genes: lack of nitrogen provoked an initial increase, followed by a marked decrease; in the cases of phosphorus and iron starvation, a general, stronger expression decrease was observed, particularly striking in the case of iron. Darkness and addition of the photosynthethic inhibitors DCMU and DBMIB also had a strong effect on gene expression. Methionine sulfoximine and azaserine, inhibitors of glutamine synthetase and glutamate synthase, respectively, provoked a sharp increase in icd expression. These results, together with previous studies, suggest that 2-oxoglutarate could be the molecule utilized by Prochlorococcus to sense the C/N balance. Besides, our results confirm the different regulation of nitrogen assimilation in Prochlorococcus with regard to other cyanobacteria.
机译：蓝细菌中涉及氮同化的五个基因，即glnA，glsF，icd，ntcA和glnB的表达，编码来自该途径的三个关键酶（谷氨酰胺合成酶，谷氨酸合酶，异柠檬酸脱氢酶）和两个调节蛋白（NtcA和PII） ，在这项工作中进行了研究。通过定量实时RT-PCR分析它们在不同条件下的变化。营养限制导致大多数研究基因的表达发生明显修饰：氮缺乏引起最初的增加，随后显着减少。在磷和铁饥饿的情况下，普遍观察到更强烈的表达下降，特别是在铁的情况下。黑暗和添加光合作用抑制剂DCMU和DBMIB对基因表达也有很强的影响。谷氨酰胺合成酶和谷氨酸合成酶的抑制剂蛋氨酸硫代肟和氮杂嘌呤分别导致icd表达急剧增加。这些结果，加上以前的研究表明，2-氧戊二酸可能是原球菌用来检测C / N平衡的分子。此外，我们的结果证实了与其他蓝细菌相比，原球菌中氮同化的调控不同。

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