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首页> 外文期刊>Fish & Shellfish Immunology >Immune responses and immune-related gene expression profile in orange-spotted grouper after immunization with Cryptocaryon irritans vaccine.
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Immune responses and immune-related gene expression profile in orange-spotted grouper after immunization with Cryptocaryon irritans vaccine.

机译：用Cryptocaryon irritans疫苗免疫后，桔斑石斑鱼的免疫应答和免疫相关基因表达谱。

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摘要

In order to elucidate the immune-protective mechanisms of inactivated Cryptocaryon irritans vaccine, different doses of C. irritans theronts were used to immunize orange-spotted grouper (Epinephelus coioides). We measured serum immobilization titer, blood leukocyte respiratory burst activity, serum alternative complement activity, and serum lysozyme activity weekly. In addition, the expression levels of immune-related genes such as interleukin-1 beta (IL-1 beta ), tumor necrosis factor- alpha (TNF- alpha ), major histocompatibility complexes I and II (MHC I and II), and transforming growth factor- beta 1 (TGF- beta 1) were determined in spleen and gills. The results showed that the immobilization titer, respiratory burst activity, and alternative complement activity of immunized fish were significantly increased, and the levels of the last two immune parameters in the high-dose vaccine group were significantly higher than in the low-dose vaccine group. Serum lysozyme activity in the high-dose vaccine group was significantly higher than in the PBS control group. Vaccination also regulated host immune-related gene expression. For example, at 2- and 3- weeks post immunization, IL-1 beta expression in the high-dose vaccine group spleen was significantly increased. At 4-weeks post immunization, the fish were challenged with a lethal dose of parasite, and the survival rates of high-dose vaccine group, low-dose vaccine group, PBS control group, and adjuvant control group were 80%, 40%, 0%, and 10% respectively. These results demonstrate that inactivated C. irritans vaccination improves specific and nonspecific immune responses in fish, enhancing their anti-parasite ability. These effects are vaccine antigen dose-dependent.
机译：为了阐明灭活隐孢子虫irritans疫苗的免疫保护机制，使用了不同剂量的衣原体C. irritans theronts来免疫橙斑石斑鱼（Epinephelus coioides）。我们每周测量一次血清固定化滴度，血液白细胞呼吸爆发活性，血清替代补体活性和血清溶菌酶活性。此外，免疫相关基因的表达水平，例如白介素-1 beta（IL-1 beta），肿瘤坏死因子-α（TNF-alpha），主要组织相容性复合物I和II（MHC I和II）以及转化测定脾脏和腮中的生长因子-β1（TGF-β1）。结果表明，高剂量疫苗组的固定化滴度，呼吸爆发活性和替代补体活性显着提高，高剂量疫苗组的后两个免疫参数水平明显高于低剂量疫苗组。高剂量疫苗组的血清溶菌酶活性显着高于PBS对照组。疫苗接种还调节宿主免疫相关基因的表达。例如，在免疫后2周和3周，高剂量疫苗组脾脏中的IL-1β表达显着增加。免疫后4周，用致死剂量的寄生虫攻击鱼，高剂量疫苗组，低剂量疫苗组，PBS对照组和佐剂对照组的存活率分别为80％，40％， 0％和10％。这些结果证明灭活的艾美氏梭菌疫苗接种可改善鱼类的特异性和非特异性免疫反应，增强其抗寄生虫能力。这些作用是疫苗抗原剂量依赖性的。

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