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首页> 外文期刊>Fish & Shellfish Immunology >Akirin2 homologues from rock bream, Oplegnathus fasciatus: Genomic and molecular characterization and transcriptional expression analysis
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Akirin2 homologues from rock bream, Oplegnathus fasciatus: Genomic and molecular characterization and transcriptional expression analysis

机译：岩鲷Oplegnathus fasciatus的Akirin2同源物：基因组和分子表征以及转录表达分析

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摘要

Akirins are conserved nuclear resident NF-kappa B signaling pathway molecules. Isoforms of akirins found in various organisms are known to play diverse roles. In this study, we have characterized two akirin2 homologues from rock bream, OfAk2(1) and OfAk2(2). The proteins derived from OfAk2(1) and OfAk2(2) revealed the presence of nuclear localization signal. Multiple sequence alignment and pairwise alignment of OfAk2(1) and OfAk2(2) with the akirin homologues, revealed high conservation and identity. Phylogenetic tree analysis revealed that the distinct position of OfAk2(1) and OfAk2(2) was close to the fish homologues and separated from the mammals and invertebrates. Genomic structure characterization revealed two distinct structures. OfAk2(1) possessed 6 exons interrupted by 5 introns whereas OfAk2(2) possessed 5 exons interrupted by 4 introns. The promoter analysis revealed the presence of significant transcription factors, which suggests its regulation by diverse stimuli. In addition, transcript expression analysis using real time quantitative reverse-transcriptase polymerase chain reaction post immune challenges with lipopolysaccharide, Edwardsiella tarda and poly I:C revealed upregulation of both OfAk2(1) and OfAk2(2) in liver, spleen and head kidney. (C) 2013 Elsevier Ltd. All rights reserved.
机译：Akirins是保守的核常驻NF-κB信号通路分子。已知在各种生物中发现的akirin异构体起着多种作用。在这项研究中，我们已经从岩鲷，OfAk2（1）和OfAk2（2）表征了两个akirin2同源物。从OfAk2（1）和OfAk2（2）衍生的蛋白质揭示了核定位信号的存在。 OfAk2（1）和OfAk2（2）的多序列比对和成对比对与akirin同源物，显示高度保守性和同一性。系统进化树分析表明，OfAk2（1）和OfAk2（2）的独特位置靠近鱼类同源物，并与哺乳动物和无脊椎动物分开。基因组结构表征揭示了两个不同的结构。 OfAk2（1）具有6个外显子，被5个内含子打断，而OfAk2（2）具有5个外显子，被4个内含子打断。启动子分析揭示了重要转录因子的存在，这表明其受到多种刺激的调节。此外，使用实时定量逆转录酶聚合酶链反应的转录表达分析在脂多糖，爱德华氏菌和聚I：C免疫攻击后揭示了肝脏，脾脏和头肾中OfAk2（1）和OfAk2（2）的上调。 （C）2013 Elsevier Ltd.保留所有权利。

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