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首页> 外文期刊>Fish & Shellfish Immunology >Trunk kidney of grass carp (Ctenopharyngodon idella) mediates immune responses against GCRV and viral/bacterial PAMPs in vivo and in vitro.
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Trunk kidney of grass carp (Ctenopharyngodon idella) mediates immune responses against GCRV and viral/bacterial PAMPs in vivo and in vitro.

机译：草鱼的躯干肾脏（Ctenopharyngodon idella）在体内和体外介导针对GCRV和病毒/细菌PAMP的免疫反应。

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Trunk kidney is a vital organ for excretion in teleosts. There have been sporadic reports of processing pathogens for the immune function in trunk kidney. However, molecular processes of pathogen recognition receptors (PRRs) responding to virus and viral/bacterial pathogen-associated molecular patterns (PAMPs) are poorly elucidated in trunk kidney. In the present study, we investigated transcriptional profiles of twelve representative immune-related genes (TLRs (TLR3, TLR7 and TLR22); RLRs (RIG-I, MDA5 and LGP2); NLRs (NOD1 and NOD2); adapter molecules (MyD88 and IPS-1); effector molecule type I interferon (IFN-I) and immunoglobulin M (IgM)) in trunk kidney tissue of grass carp (Ctenopharyngodon idella) (designated as Ci) injection of grass carp reovirus (GCRV) utilizing quantitative real-time RT-PCR (qRT-PCR). Furthermore, mRNA expression patterns of these genes (IgM excepted) were examined post GCRV infection and polyinosine-polycytidylic acid (poly(I:C)), lipopolysaccharide (LPS) or peptidoglycan (PGN) stimulation in primary trunk kidney cells of grass carp. The relative values of CiTLR3, CiTLR22 and CiMyD88 were increased post GCRV challenge and viral/bacterial PAMPs stimulation. The mRNA transcriptions of CiTLR7 were obviously activated with GCRV challenge. Remarkably, the mRNA expressions of CiRIG-I, CiMDA5, CiLGP2 and CiIPS-1 were largely up-regulated with GCRV challenge and viral/bacterial PAMPs stimulation. Interestingly, the expression tendencies of CiNOD1 and CiNOD2 were differential not only in GCRV challenge and poly(I:C) stimulation, but also in LPS and PGN stimulation. It was demonstrated that CiIFN-I induced powerful anti-viral and anti-bacterial effects in trunk kidney. In addition, the expression of CiIgM was induced at 72 h post GCRV injection in vivo. Collectively, these results suggest that trunk kidney of grass carp serves as an important immune organ, and plays crucial roles in triggering anti-viral and anti-bacterial immune responses both in vivo and in vitro.
机译：躯干肾脏是硬骨鱼排泄的重要器官。已有零星的报道涉及处理病原体以改善躯干肾脏的免疫功能。但是，在躯干肾脏中很难阐明病原体识别受体（PRRs）对病毒和病毒/细菌病原体相关分子模式（PAMPs）的响应。在本研究中，我们研究了十二种代表性免疫相关基因（TLR（TLR3，TLR7和TLR22）; RLR（RIG-1，MDA5和LGP2）; NLR（NOD1和NOD2）;衔接子分子（MyD88和IPS）的转录谱-1）;草鱼呼肠孤病毒（GCRV）注射草鱼呼肠孤病毒（GCRV）的草鱼（Ctenopharyngodon idella）（指定为Ci）的躯干肾脏组织中的I型干扰素分子（IFN-I）和免疫球蛋白M（IgM）） RT-PCR（qRT-PCR）。此外，在草鱼的初级躯干肾细胞中，在GCRV感染和polyinosine-polycydydylic acid（poly（I：C）），脂多糖（LPS）或肽聚糖（PGN）刺激后，检查了这些基因（IgM除外）的mRNA表达模式。在GCRV攻击和病毒/细菌PAMPs刺激后，CiTLR3，CiTLR22和CiMyD88的相对值增加。 CiTLR7的mRNA转录明显受到GCRV攻击的激活。值得注意的是，CiRIG-1，CiMDA5，CiLGP2和CiIPS-1的mRNA表达在GCRV攻击和病毒/细菌PAMPs刺激下大大上调。有趣的是，CiNOD1和CiNOD2的表达趋势不仅在GCRV刺激和poly（I：C）刺激中存在差异，而且在LPS和PGN刺激中也存在差异。已经证明，CiIFN-1在躯干肾脏中诱导强大的抗病毒和抗菌作用。另外，在体内GCRV注射后72小时诱导CiIgM的表达。总的来说，这些结果表明草鱼的躯干肾脏是重要的免疫器官，并且在体内和体外触发抗病毒和抗菌免疫反应中起着关键作用。

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