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首页> 外文期刊>Fish & Shellfish Immunology >Concholepas concholepas Ferritin H-like subunit (CcFer): Molecular characterization and single nucleotide polymorphism associated to innate immune response
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Concholepas concholepas Ferritin H-like subunit (CcFer): Molecular characterization and single nucleotide polymorphism associated to innate immune response

机译：孔雀菌铁蛋白H样亚基（CcFer）：与先天性免疫反应相关的分子特征和单核苷酸多态性

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摘要

Ferritin has been identified as the principal protein of iron storage and iron detoxification, playing a pivotal role for the cellular homeostasis in living organisms. However, recent studies in marine invertebrates have suggested its association with innate immune system. In the present study, one Ferritin subunit was identified from the gastropod Concholepas concholepas (CcFer), which was fully characterized by Rapid Amplification of cDNA Ends technique. Simultaneously, a challenge test was performed to evaluate the immune response against Vibrio anguillarum. The full length of cDNA Ccfer was 1030 bp, containing 513 bp of open reading frame that encodes to 170 amino acid peptide, which was similar to the Ferritin H subunit described in vertebrates. Untranslated Regions (UTRs) were identified with a 5'UTR of 244 bp that contains iron responsive element (IRE), and a 3'UTR of 273 bp. The predicted molecular mass of deduced amino acid of CcFer was 19.66 kDa and isoelectric point of 4.92. Gene transcription analysis revealed that CcFer increases against infections with V. anguillarum, showing a peak expression at 6 h post-infection. Moreover, a single nucleotide polymorphism was detected at -64 downstream 5'UTR sequence (SNP-64). Quantitative real time analysis showed that homozygous mutant allele (TT) was significantly associated with higher expression levels of the challenged group compared to wild (CC) and heterozygous (CT) variants. Our findings suggest that CcFer is associated to innate immune response in C concholepas and that the presence of SNPs may involve differential transcriptional expression of CcFer. (C) 2013 Elsevier Ltd. All rights reserved.
机译：铁蛋白已被确定为铁存储和铁解毒的主要蛋白质，对活生物体中的细胞稳态起着关键作用。但是，最近对海洋无脊椎动物的研究表明其与先天免疫系统有关。在当前的研究中，从腹足纲的Concholepas conholepas（CcFer）中鉴定出一种铁蛋白亚基，并通过cDNA末端快速扩增技术对其进行了充分表征。同时，进行攻击测试以评估针对鳗弧菌的免疫应答。 cDNA Ccfer的全长为1030 bp，包含513 bp的开放阅读框，可编码170个氨基酸的肽，这与脊椎动物中描述的Ferritin H亚基相似。用包含铁响应元件（IRE）的244bp的5'UTR和273bp的3'UTR鉴定了非翻译区（UTR）。 CcFer推导氨基酸的预测分子量为19.66 kDa，等电点为4.92。基因转录分析表明，CcFer抗鳗弧菌感染的能力增强，在感染后6小时达到峰值。此外，在-64下游5'UTR序列（SNP-64）处检测到单核苷酸多态性。实时定量分析表明，与野生型（CC）和杂合型（CT）变体相比，纯合突变体等位基因（TT）与受攻击组的更高表达水平显着相关。我们的发现表明，CcFer与C conholepas的先天免疫应答有关，并且SNP的存在可能涉及CcFer的差异转录表达。 （C）2013 Elsevier Ltd.保留所有权利。

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