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首页> 外文期刊>Fish & Shellfish Immunology >In vitro effects of prostaglandin E-2 on leucocytes from sticklebacks (Gasterosteus aculeatus) infected and not infected with the cestode Schistocephalus solidus
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In vitro effects of prostaglandin E-2 on leucocytes from sticklebacks (Gasterosteus aculeatus) infected and not infected with the cestode Schistocephalus solidus

机译：前列腺素E-2对感染和未感染solid虫血吸虫的刺背鱼（Gasterosteus aculeatus）的白细胞的体外作用

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Many helminth parasites have evolved strategies to evade the immune response of their hosts, which includes immunomodulation. Prostaglandin E-2 (PGE(2)) is one of the best-described immunomodulators in mammalian helminth parasite infections. We hypothesized that also in teleost fish anti-helminthic immune responses are regulated via PGE(2). We used a model system consisting of the tapeworm Schistocephalus solidus and its host, the three-spined stickleback (Gasterosteus aculeatus), to investigate in vitro effects of PGE(2) on head kidney leucocytes (HKL) derived from sticklebacks that were experimentally infected with S. solidus. PGE(2) was tested alone or in combination with either S. solidus antigens or bacterial lipopolysaccharides (LPS). After in vitro culture, cell viability and changes in leucocyte subpopulations (granulocytes to lymphocytes ratios) were monitored by flow cytometry and HKL were tested for their capacity to produce reactive oxygen species (ROS) with a chemiluminescence assay. In short term (2 h) HKL cultures PGE(2) did not change the total numbers of live HKL, but the production of ROS decreased significantly with high (0.1 mu mol L-1) PGE(2) concentrations. In long-term (96 h) cultures high PGE(2) concentrations induced a sharp decrease of leucocytes viability, while low (0.1 pmol L-1) and intermediate (0.1 nmol L-1) concentrations of PGE(2) caused elevated leucocyte viability compared to controls. This coincided with reduced ROS production in cultures with high PGE(2) and elevated ROS production in cultures with low PGE(2). Granulocyte to lymphocyte ratios increased with high PGE(2) concentrations alone and in combination with S. solidus antigens and LPS, most prominently with HKL from S. solidus infected sticklebacks. The present study supports the hypothesis that PGE(2) might be an immunomodulator in tapeworm fish parasite host interactions. (C) 2014 Elsevier Ltd. All rights reserved.
机译：许多蠕虫寄生虫已经进化出逃避其宿主免疫应答（包括免疫调节）的策略。前列腺素E-2（PGE（2））是哺乳动物蠕虫寄生虫感染中最能描述的免疫调节剂之一。我们假设在硬骨鱼类中，抗蠕虫免疫反应也通过PGE（2）调节。我们使用由the虫血吸虫和其宿主三棘刺背鱼（Gasterosteus aculeatus）组成的模型系统，研究了PGE（2）对源自被实验感染过刺背鱼的头肾白细胞（HKL）的体外作用。固结线虫。 PGE（2）单独或与固相链球​​菌抗原或细菌脂多糖（LPS）组合测试。体外培养后，通过流式细胞术监测细胞活力和白细胞亚群（粒细胞与淋巴细胞之比）的变化，并用化学发光法检测HKL产生活性氧（ROS）的能力。在短期（2 h）HKL培养物中，PGE（2）不会改变活HKL的总数，但是在高浓度（0.1μmolL-1）PGE（2）中，ROS的产生显着下降。在长期（96 h）培养中，高PGE（2）浓度会导致白细胞活力急剧下降，而低（0.1 pmol L-1）和中等（0.1 nmol L-1）浓度的PGE（2）会导致白细胞升高与对照相比的生存力。这与PGE（2）高的培养物中ROS的产生减少和PGE（2）低的培养物中ROS的产生增加有关。单用高浓度PGE（2）以及与固相链球​​菌抗原和LPS结合使用时，粒细胞与淋巴细胞的比例增加，最明显的是固相链球菌感染的棘背HK的HKL。本研究支持以下假设，即PGE（2）可能是tape鱼鱼寄生虫宿主相互作用中的一种免疫调节剂。 （C）2014 Elsevier Ltd.保留所有权利。

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