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首页> 外文期刊>Fish & Shellfish Immunology >p,p'-DDE depresses the immune competence of chinook salmon (Oncorhynchus tshawytscha) leukocytes.
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p,p'-DDE depresses the immune competence of chinook salmon (Oncorhynchus tshawytscha) leukocytes.

机译：p，p'-DDE会降低奇努克鲑（Oncorhynchus tshawytscha）白细胞的免疫能力。

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摘要

p,p'-DDE, the main metabolite of DDT, is still detected in aquatic environments throughout the world. Here, the effects and mechanisms by which p,p'-DDE exposure might affect the immune system of chinook salmon (Oncorhynchus tshawytscha) was studied. Isolated salmon splenic and pronephric leukocytes were incubated with different concentrations of p,p'-DDE, and cell viability, induction of apoptosis, and mitogenic responses were measured by flow cytometry and Alamar Blue assay. p,p'-DDE significantly reduced cell viability and proliferation and increased apoptosis. The effect of p,p'-DDE on pronephric leukocytes was more severe than on splenic leukocytes, likely because pronephric leukocytes had a higher proportion of granulocytes, cells that appear more sensitive to p,p'-DDE. The effect of p,p'-DDE on leukocytes appeared to vary between developmental stages or seasonal differences. The mitogenic response of leukocytes of chinook salmon exposed to p,p'-DDE in vivo exhibited a biphasic dose-response relationship. Only leukocytes isolated from salmon treated with 59 ppm p,p'-DDE had a significantly lower percentage of Ig+ blasting cells than controls, although the response was biphasic. These results support the theory that exposure to chemical contaminants could lead to an increase in disease susceptibility and mortality of fish due to immune suppression.
机译：p，p'-DDE，DDT的主要代谢产物，仍在全世界的水生环境中被检测到。在这里，研究了p，p'-DDE暴露可能影响奇努克鲑（Oncorhynchus tshawytscha）免疫系统的作用和机制。将分离的鲑鱼脾脏和前肾白细胞与不同浓度的p，p'-DDE孵育，并通过流式细胞仪和Alamar Blue检测法测量细胞活力，凋亡诱导和有丝分裂反应。 p，p'-DDE显着降低细胞活力和增殖并增加凋亡。 p，p'-DDE对肾白细胞的影响比对脾白细胞的影响更为严重，可能是因为肾白细胞的粒细胞比例更高，而这些细胞对p，p'-DDE更为敏感。 p，p'-DDE对白细胞的作用似乎在发育阶段或季节差异之间有所不同。体内暴露于p，p'-DDE的奇努克鲑鱼白细胞的促有丝分裂反应表现出两相剂量反应关系。尽管从双相反应，从59 ppm p，p'-DDE处理过的鲑鱼中分离出的白细胞中Ig +爆炸细胞的百分比明显低于对照组。这些结果支持以下理论：由于免疫抑制，接触化学污染物可能导致疾病易感性和鱼类死亡率增加。

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