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首页> 外文期刊>Fish & Shellfish Immunology >Interaction between Kazal serine proteinase inhibitor SPIPm2 and viral protein WSV477 reduces the replication of white spot syndrome virus
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Interaction between Kazal serine proteinase inhibitor SPIPm2 and viral protein WSV477 reduces the replication of white spot syndrome virus

机译：Kazal丝氨酸蛋白酶抑制剂SPIPm2与病毒蛋白WSV477之间的相互作用减少白斑综合症病毒的复制

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摘要

White spot syndrome (WSS) is a viral disease caused by white spot syndrome virus (WSSV) which leads to severe mortality in cultured penaeid shrimp. In response to WSSV infection in Penaeus monodon, a Kazal serine proteinase inhibitor SPIPm2, normally stored in the granules of granular and semi-granular hemocytes is up-regulated and found to deter the viral replication. By using yeast two-hybrid screening, we have identified a viral target protein, namely WSV477. Instead of being a proteinase, the WSV477 was reported to be a Cys2/Cys2-type zinc finger regulatory protein having ATP/GTP-binding activity. In vitro pull down assay confirmed the protein-protein interaction between rSPIPm2 and rWSV477. Confocal laser scanning microscopy demonstrated that the SPIPm2 and WSV477 were co-localized in the cytoplasm of shrimp hemocytes. Using RNA interference, the silencing of WSV477 resulted in downregulated of viral late gene VP28, the same result obtained with SPIPm2. In this instance, the SPIPm2 does not function as proteinase inhibitor but inhibit the regulatory function of WSV477. (C) 2013 Elsevier Ltd. All rights reserved.
机译：白斑综合症（WSS）是由白斑综合症病毒（WSSV）引起的病毒性疾病，导致对虾养殖中严重的死亡。响应斑节对虾中的WSSV感染，通常存储在颗粒和半颗粒血细胞颗粒中的Kazal丝氨酸蛋白酶抑制剂SPIPm2被上调，并阻止病毒复制。通过使用酵母双杂交筛选，我们已经确定了一种病毒靶蛋白，即WSV477。据报道，WSV477不是蛋白酶，而是具有ATP / GTP结合活性的Cys2 / Cys2型锌指调节蛋白。体外下拉试验证实了rSPIPm2和rWSV477之间的蛋白质-蛋白质相互作用。共聚焦激光扫描显微镜显示，SPIPm2和WSV477共定位在虾血细胞的细胞质中。使用RNA干扰，WSV477的沉默导致病毒晚期基因VP28的下调，与SPIPm2获得的结果相同。在这种情况下，SPIPm2不能充当蛋白酶抑制剂，但可以抑制WSV477的调节功能。 （C）2013 Elsevier Ltd.保留所有权利。

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