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首页> 外文期刊>Gene Expression Patterns: A Section of Mechanisms of Development >Tissue- and developmental stage-specific changes in the subcellular localization of the 26S proteasome in the ovary of Drosophila melanogaster
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Tissue- and developmental stage-specific changes in the subcellular localization of the 26S proteasome in the ovary of Drosophila melanogaster

机译：果蝇果蝇卵巢中26S蛋白酶体亚细胞定位的组织和发育阶段特异性变化。

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摘要

The intracellular localization of the 26S proteasome in the different ovarian cell types of Drosophila melanogaster was studied by means of immunofluorescence staining and laser scanning microscopy, with the use of antibodies specific for regulatory c omplex subunits or the catalytic core of the 26S proteasome. During the previtellogenic phase of oogenesis (stages 1-6), strong cytoplasmic staining was observed in the nurse cells and follicular epithelial cells, but the proteasome was not detected in t he nuclei of these cell types. The subcellular distribution of the 26S proteasome was completely different in the oocyte. Besides a constant, very faint cytoplasmic staining, there was a gradual nuclear accumulation of proteasomes during the previtelloge nic phase of oogenesis. A characteristic subcellular redistribution of the 26S proteasome occurred in the ovarian cells during the vitellogenic phase of oogenesis. There was a gradual decline in the concentration of the 26S proteasome in the nucleus of t he oocyte, and in the stage 10 oocyte the proteasome could barely be detected in the nucleus. This was accompanied by a massive nuclear accumulation of proteasomes in the follicular epithelial cells. These results demonstrate that the subcellular distrib ution of the 26S proteasome in higher eukaryotes is strictly tissue- and developmental stage-specific.
机译：通过免疫荧光染色和激光扫描显微镜研究了26S蛋白酶体在不同果蝇果蝇卵巢细胞中的细胞内定位，并使用了特异性调控复合体亚基或26S蛋白酶体催化核心的抗体。在卵胎发生前的玻璃体形成阶段（1-6阶段），在哺乳细胞和滤泡上皮细胞中观察到强烈的细胞质染色，但在这些细胞类型的细胞核中未检测到蛋白酶体。 26S蛋白酶体的亚细胞分布在卵母细胞中完全不同。除了持续的，非常微弱的细胞质染色外，在卵子发生的前玻璃体阶段，蛋白酶体的核逐渐积累。在卵子发生的卵黄形成期，卵巢细胞中发生了26S蛋白酶体的特征性亚细胞再分布。卵母细胞核中26S蛋白酶体的浓度逐渐下降，在10卵母细胞阶段，在核中几乎检测不到蛋白酶体。这伴随着卵泡上皮细胞中蛋白酶体的大量核积累。这些结果表明，在高等真核生物中26S蛋白酶体的亚细胞分布严格是组织和发育阶段特异性的。

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