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首页> 外文期刊>Fish & Shellfish Immunology >Diet related changes in non-specific immune response of European sea bass (Dicentrarchus labrax L.)
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Diet related changes in non-specific immune response of European sea bass (Dicentrarchus labrax L.)

机译：与欧洲鲈鱼（Dicentrarchus labrax L.）的非特异性免疫反应有关的饮食相关变化

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Groups of 30 sea bass (11 g) were fed diets of different protein content (55 vs 46%) and lipid (9 vs 17%) for 7 weeks (July to September). Fresh smears of bile and gall bladder were examined for Ceratomyxa ssp. and head kidney phagocytes were cultured. No other parasites or bacterial infection were detected. Respiratory burst activity and stimulatory index in 3 h adhered phagocytes were significantly higher (P<0.05) in fish fed the low lipid diet. Phagocytes, primed overnight with LPS from Salmonella typhimurium showed increased superoxide anion release in fish fed the low lipid diet. Serum lysozyme activity was decreased in the high lipid diet. The prevalence of Ceratomyxa ssp was lower in 25 fish fed the low lipid diet (28%) compared to 23 fed the high lipid diet (65.2%). Serum total protein was 64.4+3.1 mg/ml in 12 fish fed the low lipid diet compared with 73.3+- 4.2 mg/ml in fish fed the higher level of lipid. The results indicate that increase of the absolute input of dietary lipids, linked to the increase of adiposity, without significant increase of dietary protein retention has a negative effect on the immune system of European seas bass.
机译：每组30只鲈鱼（11 g）分别饲喂7周（7月至9月）的不同蛋白质含量（55％对46％）和脂质（9％对17％）的饮食。检查新鲜的胆汁和胆囊涂片中的Ceratomyxa ssp。培养头部肾脏吞噬细胞。未检测到其他寄生虫或细菌感染。喂养低脂饲料的鱼在3 h内粘附的吞噬细胞的呼吸爆发活性和刺激指数显着较高（P <0.05）。用鼠伤寒沙门氏菌的LPS引发的吞噬细胞过夜，表明低脂饮食喂养的鱼体内超氧化物阴离子的释放增加。高脂饮食使血清溶菌酶活性降低。饲喂低脂饮食的25条鱼（28％）中Ceratomyxa ssp的患病率比饲喂高脂饮食的23条鱼（65.2％）低。低脂饮食的12只鱼的血清总蛋白为64.4±3.1mg / ml，而高脂饮食的鱼的总蛋白为73.3±4.2mg / ml。结果表明，膳食脂肪绝对输入的增加与肥胖的增加有关，而饮食蛋白质的保留没有显着增加，对欧洲鲈鱼的免疫系统具有负面影响。

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