Primary culture of the hyaline haemocytes from marine decapods

Walton A; Smith VJ

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Primary culture of the hyaline haemocytes from marine decapods

机译：海洋十足动物的透明血细胞的原代培养

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This simple technique for maintaining crab hyaline haemocytes in monolayer culture for up to 14 days is based on L15 medium supplemented with 0.4M NaCl, 10% fetal calf serum and antibiotics. Viability of separated hyaline cells was 90% after 2 days,greater than80% after 7 days andgreater than70% after 14 days. The cells retained defense mechanisms as measured by phagocytosis of Psychrobacter immobilis, over the 14 days of incubation.

机译：这种在单层培养中将螃蟹透明血细胞维持长达14天的简单技术是基于补充有0.4M NaCl，10％胎牛血清和抗生素的L15培养基。分离的透明细胞的存活力在2天后为90％，在7天后大于/ 80％，在14天后大于70％。在培养的14天中，通过固定性嗜酸性杆菌的吞噬作用，细胞保留了防御机制。

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《Fish & Shellfish Immunology》 |1999年第3期|共14页
作者
Walton A; Smith VJ;
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原文格式 PDF
正文语种 eng
中图分类动物学;
关键词
in vitro culture; haemocytes; phagocytosis; Decapoda; Carcinus maenas;

机译：体外培养;血细胞;吞噬作用;十足纲;食蟹猴;

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1. Primary culture of the hyaline haemocytes from marine decapods [J] . Walton A, Smith VJ Fish & Shellfish Immunology . 1999,第3期

机译：海洋十足动物的透明血细胞的原代培养
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4. MARINE ORNAMENTAL DECAPODS - AQUACULTURE OF MITHRACULUS FORCEPS [C] . Gil Penha-Lopes, Andrew Rhyne, Junda Lin, International Conference of the European Aquaculture Society . 2004

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Primary culture of the hyaline haemocytes from marine decapods

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