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首页> 外文期刊>Fish & Shellfish Immunology >Endocytic pathway is indicated for white spot syndrome virus (WSSV) entry in shrimp
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Endocytic pathway is indicated for white spot syndrome virus (WSSV) entry in shrimp

机译：虾的白斑综合症病毒（WSSV）进入的内吞途径

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The white spot syndrome virus (WSSV) has had a serious economic impact on the global shrimp aquaculture industry in the past two decades. Although research has clarified a lot about its genome and structure, the mechanism of how WSSV enters a cell is still unclear. In this study to determine this mechanism, primary cultured hemocytes were used as an experimental model to observe the process of WSSV entry because the stable shrimp cell lines for WSSV infection are lacking. After labeling virions and endosomes with fluorescent dyes followed by observation with a confocal microscope, the results show that the WSSV colocalizes with early endosomes. Hemocytes are further treated with different endocytic inhibitors, methyl-beta-cyclodextrin (M beta CD) and chlorpromazine (CPZ). WSSV still can be detected in the hemocytes treated with CPZ, but not in the hemocytes treated with M beta CD. Thus, we conclude that WSSV adopts the caveolae-mediated endocytosis to enter the shrimp cell. (C) 2013 Elsevier Ltd. All rights reserved.
机译：在过去的二十年中，白斑综合症病毒（WSSV）对全球虾类水产养殖业产生了严重的经济影响。尽管研究已经对其基因组和结构进行了很多澄清，但是WSSV如何进入细胞的机制仍不清楚。在这项研究中，为了确定这种机制，原代培养的血细胞被用作观察WSSV进入过程的实验模型，因为缺乏稳定的虾细胞来感染WSSV。用荧光染料标记病毒体和内体后，用共聚焦显微镜观察，结果表明WSSV与早期内体共定位。用不同的内吞抑制剂，甲基-β-环糊精（MβCD）和氯丙嗪（CPZ）进一步处理血细胞。在用CPZ处理的血细胞中仍然可以检测到WSSV，但在用M beta CD处理的血细胞中不能检测到。因此，我们得出结论，WSSV采用小窝介导的内吞作用进入虾细胞。 （C）2013 Elsevier Ltd.保留所有权利。

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