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首页> 外文期刊>Fish & Shellfish Immunology >Immunoglobulin superfamily protein Dscam exhibited molecular diversity by alternative splicing in hemocytes of crustacean, Eriocheir sinensis
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Immunoglobulin superfamily protein Dscam exhibited molecular diversity by alternative splicing in hemocytes of crustacean, Eriocheir sinensis

机译：免疫球蛋白超家族蛋白Dscam通过选择性剪接甲壳动物的血细胞中的分子多样性

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摘要

Be absent of adaptive immunity which have both specificity and memory, invertebrates seem to have evolved alternative adaptive immune strategies to resist various intruding pathogens. Whereas vertebrates could generate a wide range of immunological receptors with somatic rearrangement, invertebrates possibly depend on alternative splicing of pattern-recognition receptors (PRRs). Recently, it has been suggested that a member of the immunoglobulin superfamily (IgSF), Down syndrome cell adhesion molecule (Dscam), plays a crucial role in the alternative adaptive immune system of invertebrates. At present, we successfully isolated and characterized the first crab Dscam from Eriocheir sinensis. EsDscam has typical domain architecture compared with other Dscam orthologs, including one signal-peptide, 10 immunoglobulin (Ig) domains, 6 fibronectin type III domains (FNIII), one transmembrane domain (TM) and one cytoplasmic tail. We had detected four hypervariable regions of EsDscam in the N-terminal halves of Ig2 (25) and Ig3 domain (30), the complete Ig7 (18) and also the transmembrane domain (2), potentially generate 27,000 unique isoforms at least. Transcription of EsDscam were both a) detected in all tissues, especially in immune system, digestive system and nerve system; b) significantly induced in hemocytes post lipopolysaccharides (LPS), peptidoglycans (PG) and beta-1, 3-glucans (Glu) injection. Importantly, we had detected membrane-bound and secreted Dscam isoforms in E. sinensis, and showed that secreted isoforms were extensively transcribed post different PAMPs challenge respectively. Results from immuno-localization assay revealed that EsDscam evenly distributed in the cell surface of hemocytes. These findings indicated that EsDscam is a hypervariable PRR in the innate immune system of the E. sinensis. (C) 2013 Elsevier Ltd. All rights reserved.RI Wang, Qun/I-7778-2013OI Wang, Qun/0000-0001-7929-7692
机译：由于缺乏具有特异性和记忆力的适应性免疫，无脊椎动物似乎已经进化出了替代性的适应性免疫策略来抵抗各种侵入性病原体。脊椎动物可以产生多种具有体细胞重排的免疫受体，而无脊椎动物可能取决于模式识别受体（PRR）的可变剪接。最近，已经提出免疫球蛋白超家族（IgSF）的成员，唐氏综合症细胞粘附分子（Dscam），在无脊椎动物的替代性适应性免疫系统中起着至关重要的作用。目前，我们已成功分离并鉴定了中华绒螯蟹的第一只蟹Dscam。与其他Dscam直系同源物相比，EsDscam具有典型的域结构，包括一个信号肽，10个免疫球蛋白（Ig）域，6个纤连蛋白III型域（FNIII），一个跨膜域（TM）和一个胞质尾巴。我们在Ig2（25）和Ig3结构域（30），完整Ig7（18）和跨膜结构域（2）的N末端一半中检测到EsDscam的四个高变区，可能至少产生27,000个独特的同工型。 EsDscam的转录均在以下两个方面进行：a）在所有组织中都检测到，特别是在免疫系统，消化系统和神经系统中; b）脂多糖（LPS），肽聚糖（PG）和β-1、3-葡聚糖（Glu）注射后在血细胞中得到明显诱导。重要的是，我们检测到了中华绒螯蟹中与膜结合和分泌的Dscam亚型，并表明分泌的亚型分别在不同的PAMPs攻击后被广泛转录。免疫定位测定的结果表明，EsDscam均匀分布在血细胞的细胞表面。这些发现表明，EsDscam是中华绒螯蟹先天免疫系统中的高变PRR。 （C）2013 Elsevier Ltd.保留所有权利。王RI，Qun / I-7778-2013OI Wang，Qun / 0000-0001-7929-7692

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