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首页> 外文期刊>Fish & Shellfish Immunology >Evaluation of DNA vaccination of spotted sand bass (Paralabrax maculatofasciatus) with two major outer-membrane protein-encoding genes from Aeromonas veronii.
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Evaluation of DNA vaccination of spotted sand bass (Paralabrax maculatofasciatus) with two major outer-membrane protein-encoding genes from Aeromonas veronii.

机译：用两个主要的外膜蛋白编码基因（Vereroii veronii）评估斑点鲈鱼（Paralabrax maculatofasciatus）的DNA疫苗接种。

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摘要

Genes encoding two major outer membrane proteins (OMPs) of the bacterial pathogen Aeromonas veronii, Omp38 and Omp48, were used to construct DNA vaccines. The protective effect of such vaccines against motile aeromonad septicaemia was evaluated in spotted sand bass (Paralabrax maculatofasciatus), an endemic species of the Mexican Northwest Pacific coast and a potential resource for the aquaculture industry. Weak protein expression, as determined by immunoblotting, was observed after transfection of eukaryotic cells with the DNA vaccines. Fish immunized with a single intramuscular injection of 20 micro g of the omp38 and omp48 DNA vaccines showed slightly, but significantly elevated serum antibody levels 4 and 6 weeks after vaccination, compared to fish vaccinated with the control plasmid pcDNA3.1. Spotted sand bass vaccinated with the omp38 and omp48 DNA vaccines and challenged with A. veronii by intraperitoneal route recorded a relative percent survival (RPS) between 50 and 60%. Histopathological signs of motile aeromonad septicaemia were observed in around 40% of omp38 and omp48-vaccinated fish and 80% of pcDNA3.1-vaccinated control fish. The results indicate that P. maculatofasciatus vaccinated with a single dose of DNA plasmids encoding the major OMPs from A. veronii shows partial protection against infection and mortality by A. veronii experimental infection.
机译：编码细菌病原菌维罗氏单胞菌的两个主要外膜蛋白（OMPs）的基因Omp38和Omp48用于构建DNA疫苗。在斑sand鲈（Paralabrax maculatofasciatus），墨西哥西北太平洋沿岸的特有物种和水产养殖业的潜在资源中评估了这种疫苗对运动型气单胞菌败血症的保护作用。用DNA疫苗转染真核细胞后，观察到通过免疫印迹确定的弱蛋白表达。与用对照质粒pcDNA3.1进行疫苗接种的鱼相比，单次肌内注射20微克的omp38和omp48 DNA疫苗进行免疫的鱼在接种后4周和6周显示略微但明显升高的血清抗体水平。接种了omp38和omp48 DNA疫苗并通过腹膜内途径感染维氏曲霉的斑点鲈鱼记录的相对存活率（RPS）在50％至60％之间。在约40％接种omp38和omp48的鱼和80％接种pcDNA3.1的对照鱼中观察到了运动型气单胞菌败血症的组织病理学迹象。结果表明，用单剂量的编码Ver.ii. a。veronii主要OMP的DNA质粒疫苗接种的斑节对虾显示出对Ver.ii. a。veronii实验性感染的部分保护，防止感染和致死。

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