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首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >Hepatocyte (3H)-palmitate uptake: effect of albumin surface charge modification.
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Hepatocyte (3H)-palmitate uptake: effect of albumin surface charge modification.

机译：肝细胞（3H）-棕榈酸酯摄取：白蛋白表面电荷修饰的作用。

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摘要

The role of plasma proteins on the cellular uptake of lipophilic substrates has perplexed investigators for many years. We tested the hypothesis that an ionic interaction between the protein-ligand complex and hepatocyte surface may be responsible for supplying more ligand to the cell for uptake. The surface-charged groups on albumin were modified to yield proteins having a range of isoelectric points (ALB, ALBs, ALBm, ALBe had values of 4.8-5.0, 4.5-4.7, 3.0-3.5, 8.4-8.6, respectively). [3H]-Palmitate uptake studies were performed with adult rat hepatocyte suspensions using similar unbound ligand fractions in the presence of the different binding proteins. Mass spectrometry, isoelectric focusing (pI), and heptane:water partitioning were used to determine protein molecular weight, pI, and protein-palmitate equilibrium binding constant, respectively. Hepatocyte [3H]-palmitate clearance in the presence of ALBs and ALBm were significantly lower (p < 0.05) than ALB, whereas [3H]-palmitate clearance in the presence of ALBe was significantly higher (p < 0.05) than ALB. The data were consistent with the notion that ionic interactions between extracellular protein-ligand complexes and the hepatocyte surface facilitate the uptake of long-chain fatty acids.
机译：多年来，血浆蛋白在亲脂性底物的细胞摄取中的作用使研究人员感到困惑。我们测试了蛋白质-配体复合物与肝细胞表面之间的离子相互作用可能负责为细胞提供更多配体以供摄取的假说。修饰白蛋白上的表面带电基团以产生具有等电点范围的蛋白质（ALB，ALB，ALBm，ALBe的值分别为4.8-5.0、4.5-4.7、3.0-3.5、8.4-8.6）。 [3H]-棕榈酸酯摄取研究是在存在不同结合蛋白的情况下，使用相似的未结合配体部分，对成年大鼠肝细胞悬液进行的。质谱，等电聚焦（pI）和庚烷：水分配分别用于确定蛋白质分子量，pI和蛋白质-棕榈酸酯平衡结合常数。存在ALBs和ALBm时，肝细胞[3H]-棕榈酸酯清除率显着低于（p <0.05），而存在ALBe时[3H]-棕榈酸酯清除率显着高于（p <0.05）。数据与细胞外蛋白质-配体复合物和肝细胞表面之间的离子相互作用促进长链脂肪酸摄取的观点一致。

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